›› 2009, Vol. 27 ›› Issue (3): 8-226.

• 实验研究 • Previous Articles     Next Articles

Application of Magnetic Particle Antibody Immunoassay in Detection of Anti-Schistosoma japonicum Egg Antibody

HUANG Jin1, LIU Zhen-shi2, 3, YAO Lan1, DONG Su-juan1, HE Qi-lin4, XIONG Tao4,
WEI Lan-ying1, EANG Zheng-ming1, JIANG Chang-fu1 *   

  1. 1 Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China；2 School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China；3 Beijing Bio-ekon Biotechnology Co., Ltd, Beijing 100070, China； 4 Qianjiang Institute of Schistosomiasis Control and Prevention, Qianjiang 433100, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-06-30 Published:2009-06-30
  • Contact: JIANG Chang-fu

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Abstract: Objective To establish a magnetic particle antibody immunoassay （MPAIA） for the detection of sp-ecific antibody in sera of schistosomiasis patients. Methods Fluorescein isothiocyanate （FITC） was used to label Sch-istosoma japonicum soluble egg antigen （Sj-SEA）. Anti-human IgG coated with alkaline phosphatase （ALP） as enzyme-labeled second antibody, and magnetic beads were coupled with sheep anti-FITC antibody as solid phase. Phenolphthale in monophosphate was used as substrate to set up MPAIA for the detection. Serum samples from cases with schistosomiasis or other helminth infections were tested. Results The positive rate of MPAIA was 96.7% （116/120） with the sera of S. japonicum-infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases. The positive titer with reference sample was 1 ∶ 1 600. The precision was lower than 10%. The MPAIA tips can be stored at 4 ℃ for 12 months. Conclusion MPAIA shows a high sensitivity, proper specificity and long-term validity for schistosomiasis detection.

Key words: Schistosoma japonicum, Magnetic particle antibody immunoassay, Antibody detection