›› 2009, Vol. 27 ›› Issue (2): 19-179.

• 研究简报 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the egG1Y162 Gene of Echinococcus granulosus

CAO Chun-bao1，2， MA Xiu-min1，2， DING Jian-bing1，2 *， JIA Hai-ying1，2， MAMUTY?Wulamu1，2， MA Hai-mei1，2， WEN Hao1   

  1. 1 Xinjiang Key Laboratory of Echinococcosis，The First Teaching Hospital of Xinjiang Medical University，Urumqi 830054，China；2 The College of Pre-clinic，Xinjiang Medical University，Urumqi 830011，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-04-30 Published:2009-04-30

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Abstract: A pair of primers （egG1Y162） were designed according to the nucleotide sequence of Echinococcus multilocularis emY162 antigen gene. Using genomic DNA and cDNA from protoscoleces and adult worms of E. granulosus as templates, PCR was performed with the primers to obtain fragments of egG1Y162 gene. PUCm-T/egG1Y162 rec-ombinant plasmids and PUCm-T/egY162 cDNA recombinant plasmids were constructed and identified by PCR, digestion with restriction enzyme and sequencing. The egG1Y162 antigen gene was amplified in protoscoleces and adult worms of E. granulosus. The size of the egG1Y162 gene was 1 648 bp and cDNA was 459 bp, and GenBank accession numbers were AB458258 and AB458259, respectively.

Key words: Echinococcus granulosus, egG1Y162, Cloning, Sequence analysis