›› 2009, Vol. 27 ›› Issue (1): 23-93.

• 研究简报 • Previous Articles     Next Articles

Molecular Cloning and Characterization of Ras-Homologues fromDifferent Developing Stages of Echinococcus granulosus

LV Guo-dong, WANG Jun-hua, LU Xiao-mei, WEN Hao, LIN Ren-yong*   

  1. Key Laboratory of Xinjiang Hydatid Institute，The First Teaching Hospital of Xinjiang Medical University，Urumqi 830054，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28
  • Contact: LIN Ren-yong

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Abstract: The Ras GTPase gene from protoscolex and adult worm of Echinococcus granulosus in Xinjiang were cloned by RT-PCR and named as Eg Ras-pro （GenBank No. EU560397） and Eg Ras-adult （GenBank No. EU560398）. Sequence analysis showed that each gene had 552 bp， coding 184 aa with an pI of 6.54. Bioinformatics analysis revealed that Eg Ras-pro and Eg Ras-adult had 98.4% and 98.9% homology to Echinococcus multilocularis Ras and 53.9%～78.8% homology to other species. Phylogenetic analysis showed that Eg Ras-pro and Eg Ras-adult clustered with EmRas and SmRas. The data indicated that EgRas GTPase has been expressed from protoscolex and adult worm of Echinococcus granulosus，and both are highly conservative.

Key words: Echinococcus granulosus, Ras GTPase, Sequence analysis