›› 2009, Vol. 27 ›› Issue (1): 19-82.

• 研究简报 • Previous Articles     Next Articles

Immunoscreening and Identification of Schistosoma japonicum Juvenile cDNA Library

DUAN Xin-wei1,FU Ying-hui3,LU Yan2,HUANG Cheng-yu1,JU Chuan2, XU Bin2,XU Xue-nian2,FENG Zheng2,HU Wei2 *   

  1. 1 Biotechnology School, East China University of Science and Technology, Shanghai 200237, China; 2 National Institute of Parasite Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasitis and Filariasis, Shanghai 200025, China; 3 Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

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Abstract: The cDNA library of Schistosoma japonicum （Sj） juveniles was immunoscreened with the anti-serum from day 14 post-infection mice. The inserts of the seven positive clones were sequenced and analyzed for their homology in GenBank database. Results showed that one was highly homologous to the SjHSP70 （score=650）, two were significantly homologous to the SjFABP（score=229） and Sj CDGSH-type Zn finger-containing protein-like protein（score=246）, and the other four were not homologous to genes in GenBank and thus identified as Sj novel genes. The sequences of the novel genes were submitted to GenBank and the accession numbers were obtained （EU121231, 202646, 202647 and 202648）.

Key words: Schistosoma japonicum, Schistosomula, cDNA library, Immunoscreening, Antigen gene