›› 2008, Vol. 26 ›› Issue (2): 8-118.

• 实验研究 • Previous Articles     Next Articles

Studies on Immunomodulation Effect of Recombinant Sj16 from Schistosoma japonicum on Inflammation Response of Host

SUN Xi1,2,ZHOU He-jun1,2,LV Zhi-yue1,2,ZHANG Shuang-min1,2,HU Shao-min3,ZHENG Huan-qin1,2,FENG Ming-zhao3,WU Zhong-dao1,2 *   

  1. 1 Department of Parasitology，Zhongshan School of Medicine，Sun Yat-sen University，Guangzhou 510089，China；2 Key Laboratory for Tropical Disease Control（Sun Yat-sen University），the Ministry of Education，Guangzhou 510089，China；3 Department of Biology，the Chinese University of Hong Kong，Hong Kong 730030，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-04-30 Published:2008-04-30
  • Contact: WU Zhong-dao

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Abstract: Objective To study the immunomodulation effect of the recombinationt Sj16 from Schistosoma japonicum（reSj16） on inflammation response of host. Methods reSj16 expressed in pGEX-4T-1 was purified by GST purification kit and detected by sodium dodecyl sulfate-polyacrylamide gel electropheresis （SDS-PAGE） and mass spectra （MS）. The immunomodulation effect of reSj16 was observed by means of dimethylbenzene inducing mouse ear edema model， carrageenan inducing rat voix pedis swell model and acetic acid inducing mouse experiment peritonitis model. Results The soluble protein of reSj16 was obtained and identified by SDS-PAGE and MS. reSj16 1.0，5.0，10.0 μg/kg evidently suppressed the mouse ear edema induced by dimethyl-benzene， significantly mitigated the rat voix pedis swelling induced by carrageenan， and remarkably suppressed the increase of the capillary permeability of abdominal cavity in experiment peritonitis mouse model. Conclusion The results further prove that Sj16 may be a potential immunosuppressive molecule and may have a notable effect on immunomo-dulation.

Key words: Schistosoma japonicum, reSj16, immunomodulation, carrageenan