Abstract: To prepare a large amount of pure alive Schistosoma japonicum eggs, rabbit was infected with 2000 cercariae and its liver was taken aseptically 38-45 days after infection and homogenized. The homogenate was screened through different sieves（60, 120, 200, 300, 360 meshes per inch respectively）, and washed with 1.2% NaCl. The eggs and leftover were then digested with 0.25% trypsin for 2 hours, sieved over 360 meshes per inch and washed with RPMI 1640 medium. The collected eggs reached to （95.1±6.4）% of live eggs, with a high efficiency.

Key words: Schistosoma japonicum, Egg preparation, In vitro culture