›› 2008, Vol. 26 ›› Issue (2): 10-127.

• 实验研究 • Previous Articles     Next Articles

Construction and Expression of Recombinant Baculovirus with Schistosoma japonicum SjPP Gene

YAO Li-xiao1，3， TAO Li-hong1， YANG Guan-zhen2， WU Xiang-fu2， CAI You-min1， LIN Jiao-jiao1 *   

  1. 1 Shanghai Research Center for Animal Biotechnology/Key Laboratory of Animal Parasitology of the Ministry of Agriculture/ Shanghai Institute of Veterinary Research，Chinese Academy of Agricultural Sciences，Shanghai 200232，China；2 Institute of Biochemistry and Cell Biology，Shanghai Institutes for Biological Sciences，Chinese Academy of Sciences，Shanghai 200231，China；3 Citrus Research Institute, Southwest University, Chongqing 400712，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-04-30 Published:2008-04-30
  • Contact: LIN Jiao-jiao

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Abstract: Objective To express the soluble recombinant Schistosoma japonicum SjPP proteins in TN5B1-4 cells. Methods The total RNA was extracted from adult worms of Schistosoma japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting. Results The infective recombinant baculovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells. Conclusion The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.

Key words: Schistosoma japonicum, SjPP, Bac-to-Bac Baculovirus Expression Vector System