Genotype Detection of the Merozoite Surface Protein Alleles of <EM>Plasmodium vivax</EM>

›› 2004, Vol. 22 ›› Issue (2): 6-89.

• 论著 • Previous Articles     Next Articles

Genotype Detection of the Merozoite Surface Protein Alleles of Plasmodium vivax

ZHANG Shan-ying,XU Long-shan,LU Hui-min,ZHANG Ying-zhen,GAO Qi,LI Li-sha   

  1. Fujian Institute of Parasitic Diseases;Fuzhou 350001;China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-04-30 Published:2004-04-30

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Abstract: Objective To develop a method for detecting the genotype of Plasmodium vivax merozoite surface protein 1 (PvMSP-1) alleles. Methods According to the sequence characteristic of PvMSP-1, nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to amplify the polymorphic region of ICB5-ICB6 which contains Q repeats and PvuII restriction site (Sal-1 type). The PCR product was digested by PvuII restriction endonuclease and the digested fragments were observed by 2% agarose gel electrophoresis. The allelic type was determined according to the banding pattern. Results Bands in size of 400 bp (Belem type ) and/or 470 bp (Sal-1 type ) appeared in all 98 P. vivax isolates, no band was found in negative control. After PvuII digestion, two Sal-1 type fragments (120 bp and 350 bp) were obtained from 45 samples of 470 bp. Single-band of 400 bp appeared in 3 of 40 samples with 400 bp as Belem type, two bands of 120 bp and 280 bp appeared from other 35 samples as recombination type III, and another 2 bands with 120 bp and 240 bp as Korean isolate. Conclusion The result showed that the nested PCR-RFLP may be applied in the detection and identification of the three PvMSP-1 allelic types in China.

Key words: Plasmodium vivax, membrane proteins, polymerase chain reaction, restriction fragment length polymorphism, alleles