RAPD Analysis on Different Isolates of Trichomonas vaginalis

›› 2004, Vol. 22 ›› Issue (2): 7-93.

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RAPD Analysis on Different Isolates of Trichomonas vaginalis

YUAN Li-jie,GAO Xing-zheng

Department of Biology;North China Coal Medical College;Tangshan 063000;China

Received:1900-01-01 Revised:1900-01-01 Online:2004-04-30 Published:2004-04-30

Abstract

Abstract: Objective To study genetic polymorphism of DNA on seven isolates of Trichomonas vaginalis. Methods The random amplified polymorphic DNA (RAPD) technique was performed to amplify genomic DNA of the seven T. vaginalis isolates, including Beijing 1, Beijing 2, Chengde, Tangshan, Jiujiang 1, Jiujiang 2 and Jiujiang 3. The DNA bands detected were analyzed by clustering analysis with SPSS software. Results The percentage of genetic similarity among the seven isolates was from 77.4% to (94.7%,) showing a close genetic relationship among them. The percentages between the isolates of Beijing 1 and Tangshan, Jiujiang 1 and Jiujiang 2, Beijing 2 and Jiujiang 3 were 89.2%, 92.1% and 94.7% respectively, while that of Jiujiang1 and Chengde was 77.4%, indicating a lower homology. Conclusion There are a close genetic relationship and certain gene polymorphism among the seven T. vaginalis isolates; geographical origin plays little role to the genetic characteristics.

Key words: Trichomonas vaginalis, random amplified polymorphic DNA technique

YUANLi-jie;GAOXing-zheng. RAPD Analysis on Different Isolates of Trichomonas vaginalis[J]. , 2004, 22(2): 7-93.

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RAPD Analysis on Different Isolates of Trichomonas vaginalis

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