Detection of Trichomonas vaginalis by PCR and LAMP

doi:10.12140/j.issn.1000-7423.2021.02.023

Abstract

Abstract:

To establish PCR and LAMP method for detection of Trichomonas vaginalis, the primers were synthesized, and the DNA of cultured of T. vaginalis was amplified. The feasibility of the two methods in clinical application was compared by detecting 40 T. vaginalis-positive and 40 T. vaginalis-negative samples of vaginal discharge identified by clinical microscopy. The specificity of the two methods was analyzed by amplifying DNA of Mycoplasma urealytium, Giardia lamblia, Staphylococcus aureus, Candida albicans and Escherichia coli. The sensitivity of the two methods was analyzed by determining the concentration of cultured T. vaginalis DNA after serial dilution. The results showed that PCR amplification of T. vaginalis DNA resulted in a 263-bp specific band, and the LAMP method caused a change of amplification product color from red to yellow, presenting the DNA ladder on agarose gel electrophoresis. The feasibility results showed that the coincidence rate of the 40 positive and 40 negative clinical samples by PCR were 100% and 100%, while LAMP method resulted in 100% and 97.5%, respectively. The specificity results showed that the DNA of 5 other pathogens could not be amplified by the two methods. The sensitivity test showed that the lower detection limit of the PCR method was 0.5006 ng/μl and the LAMP method 0.05006 ng/μl. These results indicate that the both methods are highly specific and the LAMP is superior to PCR in sensitivity.

Key words: Trichomonas vaginalis, PCR, LAMP

CLC Number:

R382.211

ZHAO Li-li, MIAO Xu, XIE Huan-fei, LIU Bi-han, YUAN Lin, LIN Lin, HUANG Shuang, JIANG Xu-gan, CHEN Sheng-xia, SHEN Yu-juan, CAO Jian-ping. Detection of Trichomonas vaginalis by PCR and LAMP[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(2): 260-264.

Figures/Tables 6

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