Abstract: 　Objective To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes. Methods The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software. Results Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-β-hydroxysteroid dehydrogenase(CR/20-β-HSD). Conclusion Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development.

Key words: Schistosoma japonicum, self-cure, cDNA library, immunoscreening, clone