›› 2003, Vol. 21 ›› Issue (3): 9-159.

• 论著 • Previous Articles     Next Articles

Effect of Daphnetin on SOD Activity and DNA Synthesis of Plasmodium falciparum in vitro

MU Ling-yun,WANG Qin-mei,NI Yi-chang
  

  1. Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-06-30 Published:2003-06-30

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Abstract: 　Objective To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro. Methods The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P. f) FOCI in vitro was determined with a SOD test-kit. The level of DNA synthesis of P. f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258. Results The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P. f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P. f trophozoites in synchronized in vitro culture was significantly lower than that of the control. Conclusion Daphnetin lowered SOD activity and decreased DNA synthesis of P. f in vitro.

Key words: Plasmodium falciparum, daphnetin, superoxide dismutase (SOD), deoxyribonucleic acid (DNA)