›› 2003, Vol. 21 ›› Issue (3): 10-163.

• 论著 • Previous Articles     Next Articles

cDNA Cloning and Sequence Analysis of Major Allergen of Dermatophagoides farinae Derf2 in South China

HAO Min-qi,XU Jun,ZHONG Nan-shan   

  1. Guangzhou Institute of Respiratory Diseases,Guangzhou 510120
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-06-30 Published:2003-06-30

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Abstract: 　Objective To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China. Methods cDNA of Der f 2 was cloned by RT-PCR, screened and their sequences were analyzed. Results cDNAof Der f 2 was cloned. The sequence of the cloned Der f 2 was different with that published (D10448) in GenBank, with 87 additional nucleotides inserted into the 62th nucleotide of the original one. According to the original ORF, the deduced amino acids that were located prior or after the inserted 29 amino acid sequence showed no changes. Conclusion The cDNA of Der f 2 was cloned from Dermatophagoides farinae and its sequence showed significant difference with that reported in the GenBank.

Key words: allergen, Dermatophagoides farinae, cDNA