Abstract: 　Objective To look for new candidate vaccine molecule and characterize new genes from Schistosoma japonicum. Methods New genes of S.japonicum were obtained through expressed sequence tags and analyzed on line. One interest cDNA was cloned into anti-sense eukaryotic expression vector. Results An interest cDNA JAYL0230 was obtained. Analysis showed that homology existed between its deduced amino acid sequence and defender against apoptotic death 1 of human and pig, etc. Its anti-sense cDNA was cloned into eukaryotic expression vector pEGFP-N3. Conclusion The combination of expressed sequence tags and bioinformatics is an effective way to find novel genes of creature. Construction of anti-sense eukaryotic vector is helpful in studying gene′s function.

Key words: Schistosoma japonicum, expressed sequence tags, defender against apoptotic death1Dad1, anti-sense RNA, clone