›› 2000, Vol. 18 ›› Issue (2): 3-75.

• 论著 • Previous Articles     Next Articles

CLONING OF CYSTICERCUS CELLULOSAE AgB cDNA CODING REGION

WANG Qing-min;DAI Jian-xin;ZHANG Ping-wu;SUN Shu-han
  

  1. Department of Medical Genetic;Second Military Medical University;Shanghai 200433
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-04-30 Published:2000-04-30

Abstract:  Objective] To amplify and clone Cysticercus cellulosae AgB cDNA coding region. [Methods] The AgB cDNA was amplified by RT-PCR technique from the total RNA of Cysticercus cellulosae. It was cloned into the vector pUC118 and sequenced. [Results] The PCR amplified product was a single band of 2.6 kb in size. The sequences of AgB cDNA coding region has 99.8% homology with that of Australian Cysticercus cellulosae, and their amino acid sequences have 99.3% homology. [Conclusion] Cysticercus cellulosae AgB cDNA coding region has been cloned successfully.\;

Key words: Cysticercus cellulosae gene, AgB cDNA coding region, paramyosin, PCR, gene clone.