Abstract: 　AIM: To investigate the dynamics of the anti GST antibodies in rabbits immunized with recombinant 26 kDa GST of Schistosoma japonicum . METHODS: The specific antibodies were detected weekly by GST ELISA in rabbits vaccinated with purified recombinant 26 kDa GST antigen of S.japonicum plus FCA/IFCA;rabbits immunized with 0.85% saline plus FCA/IFCA served as control group. RESULTS: The specific anti GST antibodies began to increase at week 4 post immunization,the mean OD value in rabbits vaccinated with recombinant GST could reach 1.03±0.46(adjuvant control group only 0.42±0.04).The experiment was completed at week 65 post immunization when the mean OD values for specific anti GST antibody in immunized rabbits were 0.94±0.26(0.29±0.16 in the control group). CONCLUSION:Strong anti GST antibody responses could be induced in rabbits vaccinated with recombinant 26 kDa GST of S.japonicum.

Key words: Schistosoma japonicum, recombinant antigen, glutathione S transferase, ELISA