Abstract: 　AIM∶ To construct a recombinant plasmid DNA encoding multiantigens of Plasmodium falciparum and to provide the requirements for DNA immunization. METHODS:Two oligonucleotide primers were designed to amplify MSP1 19 , the purified PCR products were digested by SalⅠ+XbaⅠ, and the recombinant plasmid pWR450 1/PfCMR was digested by EcoRⅠ+SalⅠ to recover PfCMR gene . PfCMR and MSP1 19 gene fragments were linked and recombined with mammalian expression vector pcDNA3. RESULTS:The MSP1 19 gene fragment with about 363 base pairs were specifically amplified by using PCR technique. The positive recombinant pcDNA3 PfCMR MSP1 19 (named pcDNA3 Pf8) was screened and identified by agarose gel electrophoresis,endonulease digestion and PCR technique, the whole length of Pf8 is 618 bp . CONCLUSION:By specifically amplifying MSP1 19 gene at the C terminal of MSP1,a recombinant plasmid pcDNA3 Pf8 encoding multiantigens of Plasmodium falciparum was successfully constructed.

Key words: Plasmodium falciparum, polymerase chain reaction, nucleic acid vaccine, cloning, merozoite surface protein 1