›› 1997, Vol. 15 ›› Issue (4): 193-197.

• 论著 •     Next Articles

IMMUNOSCREENING OF c DNA CLONESEXPRESSING PLASMODIUM FALCIPARUM ANTIGEN BY USING DIFFERENT ANTIBODIES

Wang Yanni1 * *; Xie Yi2; Xiao Gutian2; Li Ming1; Bi Huixiang1; Chao Sui1; Wang
Ping1; Li Yingjie1   

  1. 1 Department of Malaria Immunology, The First Military Medical University; Guangzhou 510515 ; Department of Immunology, Zunyi Medical College, Zunyi 563003 2 State Key Laboratory of Genetics and Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-08-30 Published:1997-08-30

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Abstract: AIM:To screen c DNA genes of Plasmodium falciparum(FCC/HN) and to make pre-
liminary identification.METHODS:8× 1 0 5 recombinants of Plasmodium falciparum cDNA library
were screened using rabbit immune sera in dot- ELISA;the strong- positive clones were screened
again using Mc Abs and sera from patients.Seventeenth strong- postive clones were preliminarily
identitified by PCR.RESULTS:Fifty- eight positive clones including1 7 strong- positive ones were
identified by immune sera of rabbits;eleven positive clones including 8 strong-positive ones were identified by sera of 9 patients. Nine clones were reacted with 11 strain s of 9McAbs. The inserts of 17 strong-positive clones were specifically amplified by PCR; their size ranged from ca. 300 bp-2. 5 kb. CONCLUSION: cDNA clones exp ressing F.falciparum antigen proteins were screened and would react with immune sera of rabbits, McAbs and sera of falciparum malaria patients.

Key words: Plasmodium falciparum, cDNA clone, antibody, screening