›› 2002, Vol. 20 ›› Issue (1): 12-41.

• 实验报道 • Previous Articles     Next Articles

Construction and Characterization of the cDNA Library from Schistosoma japonicum Cercariae

CHEN Xiao-guang1;LI Hua1;PENG Hong-juan1;SHEN Shu-man1;FUNG Ming-chiu2   

  1. 1 Department of Parasitology;Institute of Tropical Medicine;The First Military Medical University;Guangzhou 510515; 2 Department of Biology;The Chinese Uni
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-28 Published:2002-02-28

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Abstract: 　Objective To construct a cDNA library from Schistosoma japonicum cercariae. Methods Total RNA was extracted from 500 000 S.japonicum cercariae and used to synthesize the double-stranded cDNA and cloned into the TriplEx2 vector using "SMART cDNA Construction Kit". Results The primary titer of the constructed cDNA library is 1.8×107 pfu/ml and the titer of amplified library is 2.5×1010 pfu/ml. The average size of inserts is 1.075 kb. The recombinant efficiency is 94.4%. The full length cDNA of S.japonicum TPI and JF-2 genes was successfully amplified from the library. Conclusion A cDNA library of S.japonicum cercariae has been constructed successfully.

Key words: Schistosoma japonicum, cercariae, cDNA library, construction