Abstract: Objective To evaluate the recombinant membranous protein 29 000 （rSj29） of Schistosoma japonicum in the immunodiagnosis of schistosomiasis by indirect ELISA. Methods 394 serum samples collected from two low-endemic villages in Anhui Province were tested by rSj29-ELISA and AWA-ELISA using adult worm antigen (AWA) of S. japonicum. Meanwhile all subjects were each tested by 9 examinations with 3 fecal samples by modified Kato-Katz method, and by indirect hemagglutination assay （IHA）. Results It was found that the positive detection rates of stool examination, IHA, rSj29-ELISA and AWA-ELISA were 4.8% （19/394）, 62.2% （245/394）, 68.3% （269/394）, and 89.9%（354/394）, respectively. There was no statistical difference between rSj29-ELISA and IHA （χ²=3.2， P>0.05） with the coin-cidence of 80.7%（318/394）. The coincidence of IHA, rSj29-ELISA and AWA-ELISA with stool examination was 42.6% （168/394）, 36.0% （142/394）, and 15.0% （59/394）, respectively. Conclusion The indirect ELISA with rSj29 antigen shows similar effect as IHA for the diagnosis of schistosomiasis.

Key words: Schistosoma japonicum, Immunodiagnosis, ELISA, Indirect hemagglutination