Construction of Full-length cDNA Library ofErythrocytic Stage Plasmodium vivax

›› 2010, Vol. 28 ›› Issue (3): 18-封三.

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Construction of Full-length cDNA Library ofErythrocytic Stage Plasmodium vivax

FANG Qiang¹，XIA Hui1，YUAN Yuan-ying¹，CAO Jun²，
WANG Xue-mei¹，QI Wen-juan¹，GAO Qi^{2 *}

1 Department of Microbiology and Parasitology，Bengbu Medical College；Anhui Key Laboratory of Infection and Immunity，Bengbu 233030，China；2 Jiangsu Institute of Parasitic Diseases；Key Laboratory on Technology for Parasitic Disease Prevention and Control，Ministry of Health，China，Wuxi 214064，China

Received:1900-01-01 Revised:1900-01-01 Online:2010-06-30 Published:2010-06-30

Abstract

Abstract: 【Abstract】 Blood samples were collected from vivax malaria patients without antimalarial drug therapy. After filtration through Plasmodipur filter to remove white blood cells, Plasmodium vivax-infected RBCs were enriched by Percoll. Total RNA of P. vivax in red blood cells was isolated. A full-length cDNA library of erythrocytic stage P. vivax was constructed by the SMART cDNA library construction kit. The volume and recombinant rate of the library were evaluated. The inserted fragments were identified by PCR amplification. The titer of cDNA library was 1.14×106. The length of inserted fragment ranged from 900 to 2 500 bp, and the recombination efficiency accounted for 97.2%.

Key words: Plasmodium vivax, Erythrocytic stage, cDNA library

FANGQiang;XIAHui;YUANYuan-ying;CAOJun;WANGXue-mei;QIWen-juan;GAOQi*. Construction of Full-length cDNA Library ofErythrocytic Stage Plasmodium vivax[J]. , 2010, 28(3): 18-封三.

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Construction of Full-length cDNA Library ofErythrocytic Stage Plasmodium vivax

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