Advances in methods for detecting drug-resistance molecular markers of Plasmodium falciparum

doi:10.12140/j.issn.1000-7423.2020.04.016

Abstract

Abstract:

Malaria is a major tropical disease prevalent in Africa and Southeast Asia, which seriously threatens the health of local residents. The emergence and spread of Plasmodium falciparum resistance to anti-malaria drugs has challenged the realization of global malaria elimination goal. Current monitoring methods for P. falciparum drug resistance mainly include in vivo pharmacodynamic test, in vitro drug sensitivity test, and method of molecular markers of drug-resistance, of which the detection of molecular markers is widely used. This paper reviews research progress in the detection method using molecular markers for drug-resistance of P. falciparum resistance.

Key words: Plasmodium falciparum, Anti-malarial drugs, Molecular marker

CLC Number:

R382.31

YE Sheng-yu, CHENG Yi-yi, LI Man, ZHOU Hong-ning. Advances in methods for detecting drug-resistance molecular markers of Plasmodium falciparum[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(4): 490-495.

References 48

[1]	Cowman AF, Healer J, Marapana D, et al. Malaria: biology and disease[J]. Cell, 2016,167(3):610-624. doi: 10.1016/j.cell.2016.07.055 pmid: 27768886
[2]	Dondorp AM, Yeung S, White L, et al. Artemisinin resistance: current status and scenarios for containment[J]. Nat Rev Microbiol, 2010,8(4):272-280. doi: 10.1038/nrmicro2331 pmid: 20208550
[3]	Xu C, Huang BC, Yan G, et al. Research progress on molecular markers of Plasmodium falciparum and drug resistance-related molecules[J]. Chin J Pathog Biol, 2016,11(12):1149-1152. (in Chinese)
	( 徐超, 黄炳成, 闫歌, 等. 恶性疟原虫与耐药性相关分子遗传标记的研究进展[J]. 中国病原生物学杂志, 2016,11(12):1149-1152.)
[4]	World Health Organization. Guidelines for the treatment of malaria[J]. Geneva: WHO, 2015.
[5]	Noedl H, Se Y, Schaecher K, et al. Evidence of artemisinin-resistant malaria in western Cambodia[J]. N Engl J Med, 2008,359(24):2619-2620. doi: 10.1056/NEJMc0805011 pmid: 19064625
[6]	Li N, Huang YM, Cai WB, et al. Advances in the study of the sensitivity of Plasmodium falciparum to dihydroartemisinin-piperaquine[J]. Chin J Pathog Biol, 2017,12(10):1025-1027. (in Chinese)
	( 李娜, 黄亚铭, 蔡文斌, 等. 恶性疟原虫对双氢青蒿素-哌喹敏感性研究进展[J]. 中国病原生物学杂志, 2017,12(10):1025-1027.)
[7]	Dondorp AM, Nosten F, Yi P, et al. Artemisinin resistance in Plasmodium falciparum malaria[J]. N Engl J Med, 2009,361(5):455-467. doi: 10.1056/NEJMoa0808859 pmid: 19641202
[8]	Zhang YL, Pan WQ. Research progress on the resistance of Plasmodium falciparum to artemisinin[J]. Chin J Parasitol Parasit Dis, 2015,33(6):418-424. (in Chinese)
	( 张逸龙, 潘卫庆. 恶性疟原虫对青蒿素产生抗性的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2015,33(6):418-424.)
[9]	Ye R, Hu D, Zhang Y, et al. Distinctive origin of artemisinin resistant Plasmodium falciparum on the China-Myanmar border[R]. Sci Rep, 2016,6:20100.
[10]	Zhao SM, Wang MY. Global status and basic research on the resistance of Plasmodium falciparum to artemisinin[J]. Chin J Parasitol Parasit Dis, 2014,32(5):380-384. (in Chinese)
	( 赵绍敏, 王满元. 恶性疟原虫对青蒿素类药物产生耐药性的全球现状和基础研究[J]. 中国寄生虫学与寄生虫病杂志, 2014,32(5):380-384.)
[11]	Ariey F, Witkowski B, Amaratunga C, et al. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria[J]. Nature, 2014,505(7481):50-55. doi: 10.1038/nature12876 pmid: 24352242
[12]	Plowe CV. The evolution of drug-resistant malaria[J]. Trans R Soc Trop Med Hyg, 2009,103(Suppl 1):S11-S14. doi: 10.1016/j.trstmh.2008.11.002
[13]	Lin JT, Juliano JJ, Wongsrichanalai C. Drug-resistant malaria: the era of ACT[J]. Curr Infect Dis Rep, 2010,12(3):165-173. doi: 10.1007/s11908-010-0099-y
[14]	Noedl H, Attlmayr B, Wernsdorfer WH, et al. A histidine-rich protein 2-based malaria drug sensitivity assay for field use[J]. Am J Trop Med Hyg, 2004,71(6):711-714. pmid: 15642959
[15]	Zhang MH, Lu F, Cao J, et al. Research progress on molecular markers related to drug resistance of Plasmodium falciparum[J]. Chin J Schisto Control, 2015,27(3):323-327. (in Chinese)
	( 张梅花, 陆凤, 曹俊, 等. 恶性疟原虫药物抗性相关分子标记研究进展[J]. 中国血吸虫病防治杂志, 2015,27(3):323-327.) doi: 10.16250/j.32.1374.2014227
[16]	Fidock DA, Nomura T, Talley AK, et al. Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance[J]. Mol Cell, 2000,6(4):861-871. doi: 10.1016/s1097-2765(05)00077-8 pmid: 11090624
[17]	Fidock DA, Eastman RT, Ward SA, et al. Recent highlights in antimalarial drug resistance and chemotherapy research[J]. Trends Parasitol, 2008,24(12):537-544. doi: 10.1016/j.pt.2008.09.005 pmid: 18938106
[18]	Awasthi G, Das A. Genetics of chloroquine-resistant malaria: a haplotypic view[J]. Mem Inst Oswaldo Cruz, 2013,108(8):947-961. doi: 10.1590/0074-0276130274 pmid: 24402147
[19]	Lakshmanan V, Bray PG, Verdier-Pinard D, et al. A critical role for PfCRT K76T in Plasmodium falciparum verapamil-reversible chloroquine resistance[J]. EMBO J, 2005,24(13):2294-2305. pmid: 15944738
[20]	Dai TT, Lu CC, Zheng XB. Application research progress of Loop-mediated isothermal amplification in the pathogenic microorganism[J]. J Nanjing Agric Univ, 2015,38(5):695-703. (in Chinese)
	( 戴婷婷, 陆辰晨, 郑小波. 环介导等温扩增技术在病原物检测上的应用研究进展[J]. 南京农业大学学报, 2015,38(5):695-703.)
[21]	Chahar M, Mishra N, Anvikar A, et al. Establishment and application of a novel isothermal amplification assay for rapid detection of chloroquine resistance (K76T) in Plasmodium falciparum[J]. Sci Rep, 2017,7:41119. doi: 10.1038/srep41119 pmid: 28134241
[22]	Lin LH, Huang LX, Liu GM. Value and feasibility of improved loop-mediated isothermal amplification technique in the detection of SNPs of Plasmodium falciparum resistance gene[J]. Hainan Med J, 2017,28(15):2474-2477. (in Chinese)
	( 林岭海, 黄良喜, 刘光明. 改良环介导等温扩增技术在疟原虫耐药基因SNP检测中的价值及可行性[J]. 海南医学, 2017,28(15):2474-2477.)
[23]	Bashir IM, Otsyula N, Awinda G, et al. Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of Plasmodium events and prediction of sick visits during a malaria vaccine study[J]. PLoS One, 2013,8(3):e56828. doi: 10.1371/journal.pone.0056828 pmid: 23554856
[24]	Farcas GA, Soeller R, Zhong K, et al. Real-time polymerase chain reaction assay for the rapid detection and characterization of chloroquine-resistant Plasmodium falciparum malaria in returned travelers[J]. Clin Infect Dis, 2006,42(5):622-627. doi: 10.1086/500134 pmid: 16447106
[25]	Keen J, Farcas GA, Zhong K, et al. Real-time PCR assay for rapid detection and analysis of PfCRT haplotypes of chloroquine-resistant Plasmodium falciparum isolates from India[J]. J Clin Microbiol, 2007,45(9):2889-2893. doi: 10.1128/JCM.02291-06 pmid: 17609321
[26]	Rolf HA, Emmelien A, Anja R, et al. High-resolution melting analysis(HRMA): more than just sequence variant screening[J]. Hum Mutat, 2009,30(6):860-866. doi: 10.1002/humu.21019 pmid: 19418555
[27]	Andriantsoanirina V, Lascombes V, Ratsimbasoa A, et al. Rapid detection of point mutations in Plasmodium falciparum genes associated with antimalarial drugs resistance by using high-resolution melting analysis[J]. J Microbiol Methods, 2009,78(2):165-170. doi: 10.1016/j.mimet.2009.05.013 pmid: 19465064
[28]	Gan LS, Loh JP. Rapid identification of chloroquine and atovaquone drug resistance in Plasmodium falciparum using high-resolution melt polymerase chain reaction[J]. Malar J, 2010,9:134. doi: 10.1186/1475-2875-9-134 pmid: 20487570
[29]	Dong Y, Zhang ZX, Adagu IS. Detection of polymorphism of Plasmodium falciparum chloroquine resistant gene 76 Codon by nested PCR/RFLP[J]. J Pract Parasit Dis, 2001(3):104-107. (in Chinese)
	( 董莹, 张再兴, Adagu IS. 套式PCR检测恶性疟原虫Pfcrt基因76号编码多态性及RFLP分析[J]. 实用寄生虫病杂志, 2001(3):104-107.)
[30]	Pholwat S, Liu J, Stroup S, et al. The malaria TaqMan array card includes 87 assays for Plasmodium falciparum drug resistance, identification of species, and genotyping in a single reaction[J]. Antimicrob Agents Chemother, 2017,61(5):e00110-e00117. doi: 10.1128/AAC.00110-17 pmid: 28264857
[31]	Crameri A, Marfurt J, Mugittu K, et al. Rapid microarray-based method for monitoring of all currently known single-nucleotide polymorphisms associated with parasite resistance to antimalaria drugs[J]. J Clin Microbiol, 2007,45(11):3685-3691. doi: 10.1128/JCM.01178-07 pmid: 17804664
[32]	Wong RP, Karunajeewa H, Mueller I, et al. Molecular assessment of Plasmodium falciparum resistance to antimalarial drugs in Papua New Guinea using an extended ligase detection reaction fluorescent microsphere assay[J]. Antimicrob Agents Chemother, 2011,55(2):798-805. doi: 10.1128/AAC.00939-10 pmid: 21078925
[33]	Mohon AN, Menard D, Alam MS, et al. A novel single-nucleotide polymorphism loop mediated isothermal amplification assay for detection of artemisinin-resistant Plasmodium falciparum malaria[J]. Open Forum Infect Dis, 2018, 5(4): ofy011. doi: 10.1093/ofid/ofy011 pmid: 29707598
[34]	Singh R, Singh DP, Savargaonkar D, et al. Evaluation of SYBR green I based visual loop-mediated isothermal amplification (LAMP) assay for genus and species-specific diagnosis of malaria in P. vivax and P. falciparum endemic regions[J]. J Vector Borne Dis, 2017,54(1):54-60. pmid: 28352046
[35]	Imai K, Tarumoto N, Misawa K, et al. A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION^TM nanopore sequencer[J]. BMC Infect Dis, 2017,17(1):621. doi: 10.1186/s12879-017-2718-9 pmid: 28903726
[36]	Imai K, Tarumoto N, Runtuwene LR, et al. An innovative diagnostic technology for the Codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer[J]. Malar J, 2018,17(1):217. doi: 10.1186/s12936-018-2362-x pmid: 29843734
[37]	Malpartida-Cardenas K, Rodriguez-Manzano J, Yu LS, et al. Allele-specific isothermal amplification method using unmodified self-stabilizing competitive primers[J]. Anal Chem, 2018,90(20):11972-11980. doi: 10.1021/acs.analchem.8b02416 pmid: 30226760
[38]	Vachot-Ganée L, Khim N, Iannello A, et al. A novel field-based molecular assay to detect validated artemisinin-resistant k13 mutants[J]. Malar J, 2018,17(1):175. doi: 10.1186/s12936-018-2329-y pmid: 29690890
[39]	Mens PF, van Overmeir C, Bonnet M, et al. Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum[J]. Malar J, 2008,7:48. doi: 10.1186/1475-2875-7-48 pmid: 18346279
[40]	Cruz RE, Shokoples SE, Manage DP, et al. High-throughput genotyping of single nucleotide polymorphisms in the Plasmodium falciparum dhfr gene by asymmetric PCR and melt-curve analysis[J]. J Clin Microbiol, 2010,48(9):3081-3087. doi: 10.1128/JCM.00634-10 pmid: 20631115
[41]	Kamau E, Alemayehu S, Feghali KC, et al. Development of a TaqMan Allelic Discrimination assay for detection of single nucleotides polymorphisms associated with anti-malarial drug resistance[J]. Malar J, 2012,11:23. doi: 10.1186/1475-2875-11-23 pmid: 22264294
[42]	Yongkiettrakul S, Kampeera J, Chareanchim W, et al. Simple detection of single nucleotide polymorphism in Plasmodium falciparum by SNP-LAMP assay combined with lateral flow dipstick[J]. Parasitol Int, 2017,66(1):964-971. doi: 10.1016/j.parint.2016.10.024 pmid: 27816495
[43]	Daniels R, Ndiaye D, Wall M, et al. Rapid, field-deployable method for genotyping and discovery of single-nucleotide polymorphisms associated with drug resistance in Plasmodium falciparum[J]. Antimicrob Agents Chemother, 2012,56(6):2976-2986. doi: 10.1128/AAC.05737-11 pmid: 22430961
[44]	Bass C, Nikou D, Donnelly MJ, et al. Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods[J]. Malar J, 2007,6:111. doi: 10.1186/1475-2875-6-111 pmid: 17697325
[45]	Reed MB, Saliba KJ, Caruana SR, et al. Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum[J]. Nature, 2000,403(6772):906-909. pmid: 10706290
[46]	Ibraheem ZO, Abd Majid R, Noor SM, et al. Role of different pfcrt and pfmdr-1 mutations in conferring resistance to antimalaria drugs in Plasmodium falciparum[J]. Malar Res Treat, 2014,2014:950424. doi: 10.1155/2014/950424 pmid: 25506039
[47]	Purfield A, Nelson A, Laoboonchai A, et al. A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR[J]. Malar J, 2004,3:9. doi: 10.1186/1475-2875-3-9 pmid: 15132750
[48]	Nankoberanyi S, Mbogo GW, LeClair NP, et al. Validation of the ligase detection reaction fluorescent microsphere assay for the detection of Plasmodium falciparum resistance mediating polymorphisms in Uganda[J]. Malar J, 2014,13:95. doi: 10.1186/1475-2875-13-95 pmid: 24629020