Experimental observation of the development of Trichinella spiralis muscle larvae in mice

doi:10.12140/j.issn.1000-7423.2019.02.022

Abstract

Abstract:

To observe the developmental process and characteristics of Trichinella spiralis muscle larvae in mice, 28 female Kunming mice were orally infected with 100 T. spiralis muscle larvae and each two mice were euthanized at 8, 9, 10, 11, 13, 17, 20, 23, 27, 29, 50, 60, 240 and 400 days post infection. The diaphragm and partial abdominal muscles were collected, sectioned and stained with alum carmine acetate staining. The muscle larvae, larval capsules were observed under microscope. The muscle larvae were found in the diaphragm as early as 10 days post infection (10 d) and in abdominal muscles at 13 d, mostly with straight shape. The larvae were shaped in curve, circle, folded and larval capsules were formed at 17 d. The capsular bags were well developed, the stripes were seen in the wall of the capsule, and the capsule structure was divided into two layers without clear boundary at 29 d. The larvae twisted into a mass, and the structure inside the capsule began to appear transparent at 60 d. The capsule started to degenerate and the wall of the capsule began to appear transparent at 400 d. The average size of muscle larvae was (131.7 ± 21.4) μm × (10.4 ± 2.5) μm at 10 d, (435.5 ± 201.5) μm × (22.8 ± 5.2) μm at 17 d, (928.4 ± 188.5) μm × (35.0 ± 5.2) μm at 29 d and (1044.4 ± 86.7) μm × (38.3 ± 3.0) μm at 60 d, with significant difference in size between each developmental time points (P ﹤ 0.05 or P ﹤ 0.01). According to the experimental results, larval and capsular development can be roughly divided into four stages: pre-encapsulated larvae, larvae with capsular development, larvae with mature capsular bag, and larvae with degraded capsular bag. The morphological structure of larvae and capsules is closely related to the time of infection.

Key words: Trichinella spiralis, Muscle larvae, Staining, Examination

CLC Number:

R383.15

Guo-ying WANG, Xiang-hui LI. Experimental observation of the development of Trichinella spiralis muscle larvae in mice[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(2): 235-237.

感染时间/d	长/μm	宽/μm
8	-	-
9	-	-
10	131.7 ± 21.4	10.4 ± 2.5
11	259.6 ± 73.6	15.8 ± 2.8
13	360.5 ± 95.7	21.8 ± 3.5
17	435.5 ± 201.5	22.8 ± 5.2
20	771.2 ± 190.2	30.5 ± 5.8
23	744.8 ± 292.3	32.2 ± 7.9
27	919.1 ± 164.6	37.5 ± 5.8
29	928.4 ± 188.5	35.0 ± 5.2
50	1 039.8 ± 90.8	37.3 ± 3.1
60	1 044.4 ± 86.7	38.3 ± 3.0
240	1 030.9 ± 100.2	36.6 ± 3.3
400	1 058.3 ± 105.4	38.5 ± 2.8

[1]	TANG Qi, LV Chao, WANG Xi, GUO Suying, XU Xiaojuan, ZHU Hai, LI Yinlong, LIN Weina, ZHOU Xinjie, FENG Ting, XU Jing, QIN Zhiqiang. Efficiency evaluation of three assays for detection of Schistosoma japonicum infections in wild mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 186-191.
[2]	XIONG Ye, HU Chen. Diagnosis of a case with scabies in a primary healthcare institution [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 301-303.
[3]	SUN Li, LU Enchang, CHENG Meng, AI Yanhong. A case report of hepatic cystic echinococcosis with a latent infection period of 25 years [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(6): 817-819.
[4]	LI Rui, YU Yingying, ZHUO Wanjun, YANG Shouwang, CHEN Linbi, HUANG Yishu. A case of imported schistosomiasis haematobia from a foreigner [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 547-549.
[5]	LUO Zeni, WU Anqi, WANG Zhikai, PAN Jin, SUN Ximeng. Protective effect of IL-4 to Trichinella spiralis infection in the intestinal phase [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 354-359.
[6]	LIU Yi, CAI Yuchun, CHEN Jiaxu, CHEN Shaohong, YU Yingfang. Isolation, identification, and omics analysis of extracellular vesicles from Trichinella spiralis newborn larvae [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 225-233.
[7]	HUANG Mengyu, CHEN Dikun, CHEN Huiru, FAN Mengpei, LIU Junyou, QUAN Yunfan. Investigation on Angiostrongylus cantonensis infection in Achatina fulica in Haikou wetland park [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 640-643.
[8]	ZHANG Xu, SUN Ximeng. Research progress on the immune evasion mechanism in Trichinella spiralis infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 492-496.
[9]	HAO Huinan, CHENG Yongkang, ZHANG Ru, HAN Lulu, SONG Yanyan, LONG Shaorong, LIU Ruodan, ZHANG Xi, WANG Zhongquan, CUI Jing. Immunoproteomic analysis on the soluble antigens of Trichinella spiralis newborn larvae [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 176-182.
[10]	ZHANG Yaoguang, JIANG Li, WANG Zhenyu, ZHU Min, ZHU Qian, MA Xiaojiang, YU Qing, Chen Jian. Analysis of the causes of misdiagnosis of seven imported malaria cases in Shanghai from 2020 to 2021 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 68-74.
[11]	SUN Ling-cong, WU Dong-ni, DONG Xiao-rong, YI Jia, TU Zhen, LIN Wen, ZHU Hong, ZHOU Xiao-rong. Evaluation of the testing ability of malaria laboratories in Hubei Province from 2016 to 2020 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(5): 611-616.
[12]	CAI Zi-han, CAO Ying, ZHU Feng-long, LI Qian, HE Yan-hong, YANG Yi-mei. Application of gold nanorod labeling in diagnosis of Trichinella spiralis infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(5): 652-658.
[13]	ZHANG Yao-guang, JIANG Li, WANG Zhen-yu, ZHU Min, ZHU Qian, MA Xiao-Jiang, WU Huan-yu. Laboratory diagnosis of two misdiagnosed imported Plasmodium ovale malaria cases in Shanghai [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(4): 553-556.
[14]	ZHANG Yao-guang, JIANG Li, WANG Zhen-yu, ZHU Min, ZHU Qian, MA Xiao-jiang, WU Huan-yu. Analysis of malaria detection capability of laboratories in the districts of Shanghai during 2017—2019 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(3): 337-342.
[15]	WANG Guo-ying, CHEN Dan-dan, ZHENG Xue-li, LI Xiang-hui, ZHANG Hao, ZHANG Jun, TENG Tie-shan. Relationship between the infectivity of encysted larvae and the developmental stage of Trichinella spiralis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(6): 785-788.

Experimental observation of the development of Trichinella spiralis muscle larvae in mice

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 2

References 10

Related Articles 15

Recommended Articles

Metrics

Comments