Effect of met-enkephalin on phagocytosis of Oncomelania hupensis hemocytes

doi:10.12140/j.issn.1000-7423.2019.02.023

CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2019, Vol. 37 ›› Issue (2): 238-241.doi: 10.12140/j.issn.1000-7423.2019.02.023

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Effect of met-enkephalin on phagocytosis of Oncomelania hupensis hemocytes

Xiang-hong WEI^1,²(), Ping TAN^1,^2,^*(), Jin-hua WAN^1,², Yan-bing SUN³, Shi-wen LE³, Qian-wen TIAN³, Na LIU³, Han-bing BAO³, Yue SHEN³

1 Department of Pathogenic Biology, School of Medicine, Wuhan University of Science and Technology,Wuhan 430065,China
2 Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan 430065,China
3 Class 2016, School of Medicine, Wuhan University of Science and Technology,Wuhan 430065,China

Received:2018-09-27 Online:2019-04-30 Published:2019-05-13
Contact: Ping TAN E-mail:996079380@qq.com;ptan61@qq.com
Supported by:
Supported by Hubei Province Health and Family Planning Scientific Research Project（No. WJ2017X014）

Abstract

Abstract:

To observe the effect of Met-enkephalin (MENK) on phagocytosis of hemocytes of Oncomelania hupensis, the hemolymph was obtained by squeezing the soft tissues of O. hupensis. The hemocytes in the hemolymph were incubated with different concentrations of MENK (0, 1, 5 and 10 mg/L) in the presence or absence of formaldehyde-treated sheep erythrocyte (FSE) at room temperature for 60 min. The hemocytes were stained with acridine orange and observed under fluorescence microscope. The results showed that the low concentration of MENK (1 mg/L) significantly stimulated the development of pseudopodia in the treated hemocytes of O. hupensis. The rates of pseudopodia development in the group treated with 1 mg/L of MENK was 48.77% (without FSE) and 48.3% (with FSE) with statistical significance compared to the control group (39.99%)(P < 0.01). The low concentration of MENK also stimulated the phagocytosis of treated hemocytes to FSE (48.77%) compared to control group without treatment (40.09%) with significant difference (P < 0.01). However, the high concentration of MENK (5 mg/L or 10 mg/L) not only inhibited hemocytes of Oncomelania to develop pseudopodia [27.04% (5 mg/L), 26.29% (5 mg/L + FSE) , 19.23% (10 mg/L) and 18.3%(10 mg/L + FSE) with significant difference compared to control group(χ² = 99.366, 109.74, 244.446, 274.34, respectively, all P < 0.01), but also dramatically inhibited the phagocytosis rate of treated hemocytes to FSE[7.37% (5 mg/L) and 5.8% (10 mg/L)] compared to 40.09% in untreated control group with statistical significance (P < 0.01). The results demonstrate that low concentrations of MENK can stimulate, but high concentration of MENK can inhibit the phagocytosis of treated hemocytes of O. hupensis.

Key words: Oncomelania hupensis, Hemocytes, Met-enkephalin, Acridine orange

CLC Number:

R383.241

Xiang-hong WEI, Ping TAN, Jin-hua WAN, Yan-bing SUN, Shi-wen LE, Qian-wen TIAN, Na LIU, Han-bing BAO, Yue SHEN. Effect of met-enkephalin on phagocytosis of Oncomelania hupensis hemocytes[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(2): 238-241.

Figures/Tables 2

References 18

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组别	圆形细胞				伸展型细胞		梭形细胞		计数细胞总数	吞噬醛化绵羊红细胞的伸展细胞数	伸展细胞吞噬醛化绵羊红细胞率/%
	红色胞浆		绿色胞浆		伸展型细胞		梭形细胞
	数量	百分比/%	数量	百分比/%	数量	百分比/%	数量	百分比/%
A	931	34.73	588	21.93	1 072	39.99	90	3.36	2 681
B	903	33.26	607	22.36	1 101	40.55	104	3.83	2 715
C	772	28.46	548	20.20	1 323	48.77	70	2.58	2 713
D	927	35.50	888	34.01	706	27.04	90	3.45	2 611
E	882	40.20	841	38.33	422	19.23	49	2.23	2 194
F	777	35.05	481	21.70	878	39.60	81	3.65	2 217	352	40.09
G	823	29.73	533	19.26	1 337	48.30	75	2.71	2 768	652	48.77
H	868	34.32	926	36.62	665	26.29	70	2.77	2 529	49	7.37
I	941	41.60	863	38.15	414	18.30	44	1.95	2 262	24	5.80

Effect of met-enkephalin on phagocytosis of Oncomelania hupensis hemocytes

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