Morphological observation and phenotypic detection of dendritic cells from peripheral blood of patients with alveolar echinococcosis

Abstract

Abstract:

Objective To understand the morphological and phenotypic characteristics of dendritic cells (DCs) from peripheral blood of patients with alveolar echinococcosis. Methods Peripheral blood was collected from 10 patients with alveolar echinococcosis (AE), 10 healthy Han participants (HH) (n = 10) and 10 healthy Tibetan participants (TH), respectively. Monocytes were isolated, and cultured by adherent culture. The monocytes were induced into DCs with recombinant human granulocyte macrophage colony stimulating factor and recombinant human interleukin 4. DCs were collected on days 1, 3, 5 and 7 of culture and cell morphology was observed by inverted microscopy and scanning electron microscopy. The positive rate of cell surface markers was detected by flow cytometry on day 7. Data were analyzed with SPSS 22.0 software. Results On day 1 of in vitro induction, the majority of DCs in the 3 groups had a single round shape with clear boundary, bright cytoplasm, and a small volume, floating in the culture medium. On day 3 of induction, the three groups of DCs aggregated into semi-suspension and the cells enlarged with an irregular shape. On day 5 of induction, the three groups of DCs formed colonies, and most DCs had a rough surface. On day 7 of induction, the three groups of DCs grew into semi-suspension with a filamentous cell edges. Compared to the HH and TH groups, the number of DC colonies in group AE was smaller, and the irregular protuberances of DC were not obvious, displaying an atypical DC morphology. The positive expression rates of CD1a, CD80 and CD86 on the surface of DCs induced differentiation in AE group (12.73% ± 1.73%, 12.41% ± 2.83% and 16.34% ± 3.59%, respectively) were significantly lower than those in the HH and TH groups (P < 0.05), while those of HH group (18.40% ± 1.20%, 20.77% ± 3.40%, 31.78% ± 5.02%) and TH group(17.50% ± 1.44%, 23.75% ± 5.33%, 33.20% ± 2.47%) were not significantly different (P > 0.05). Conclusion The induced DCs in the AE group have an atypical morphology and a decreased level of cell surface markers, indicating the occurrence of maturation disorder.

Key words: Alveolar echinococcosis, Dendritic cell, Cell morphology, Immunophenotype

CLC Number:

R532.32

Yong FU, Ru MENG, Hai-fang XUE, Hai-ning FAN, Hai-feng NIU, Zi-jia ZHOU, Hong-bin WANG. Morphological observation and phenotypic detection of dendritic cells from peripheral blood of patients with alveolar echinococcosis[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(5): 474-477.

Figures/Tables 3

References 12

[1]	Kawamura N, Kamiyama T, Sato N, et al. Long-term results of hepatectomy for patients with alveolar echinococcosis: a single-center experience[J]. J Am Coll Surg, 2011, 212(5): 804-812.
[2]	韩秀敏, 王虎, 蔡辉霞, 等. 青海省达日县棘球蚴病流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(1): 22-26.
[3]	Yapici O, Erturk SM, Ulusay M, et al. Hepatic alveolar echinococcosis: a diagnostic challenge[J]. JBR-BTR, 2011, 94(1): 21-23.
[4]	McManus DP, Li Z, Yang S, et al. Case studies emphasising the difficulties in the diagnosis and management of alveolar echinococcosis in rural China[J]. Parasit Vectors, 2011, 4: 196.
[5]	Siracusano A, Delunardo F, Teggi A, et al. Cystic echinococcosis: aspects of immune response, immunopathogenesis and immune evasion from the human host[J]. Endocr Metab Immune Disord Drug Targets, 2012, 12(1): 16-23.
[6]	Manicassamy S, Pulendran B.Dendritic cell control of tolerogenic responses[J]. Immunol Rev, 2011, 241(1): 206-227.
[7]	Riganò R, Buttari B, Profumo E, et al. Echinococcus granulosus antigen B impairs human dendritic cell differentiation and polarizes immature dendritic cell maturation towards a Th2 cell response[J]. Infect Immun, 2007, 75(4): 1667-1678.
[8]	张静宵, 马霄, 刘玉芳, 等. 青海省棘球蚴病流行与分布情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(5): 460-465.
[9]	张梦媛, 王立英, 官亚宜, 等. 棘球蚴病严重程度的Meta分析[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(2): 156-160.
[10]	Meierhofer D, Mayr JA, Foetschl U, et al. Decrease of mitochondrial DNA content and energy metabolism in renal cell carcinoma[J]. Carcinogenesis, 2004, 25(6): 1005-1010.
[11]	刘弓伯, 邵英梅, 单骄宇, 等. 囊性包虫病患者外周血单核细胞诱导的树突状细胞形态及表型特点[J]. 中国病原生物学杂志, 2010, 5(3): 177-179.
[12]	Riganò R, Buttari B, De Falco E, et al. Echinococcus granulosus-specific T-cell lines derived from patients at various clinical stages of cystic echinococcosis[J]. Parasite Immunol, 2004, 26(1): 45-52.

实验分组Experimental group	DCs表面标志分子阳性表达率/% Positive expression rate of surface molecules on dendritic cells/%
实验分组Experimental group	CD1a	CD80	CD86
汉族健康志愿者Healthy Han volunteers	18.40 ± 1.20	20.77 ± 3.40	31.78 ± 5.02
藏族健康志愿者Healthy Tibetan volunteers	17.50 ± 1.44	23.75 ± 5.33	33.20 ± 2.47
多房棘球蚴病患者Alveolar echinococcosis patients	12.73 ± 1.73^a	12.41 ± 2.83^a	16.34 ± 3.59^a

[1]	KUI Yan, XUE Chuizhao, WANG Xu, LIU Baixue, WANG Ying, WANG Liying, YANG Shijie, HAN Shuai, XU Xuenian. Progress of echinococcosis control in China, 2022 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 8-16.
[2]	ZHU Aiya, WANG Xu, WANG Jiangyou, WANG Ying, LI Yang, SONG Shan, GENG Yan, LAN Ziyao, DAI Jiarui. A child case of alveolar echinococcosis in Guizhou Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 520-523.
[3]	YE Jingming, HE Wei, LIU Huiyuan, YU Xiao, LUO Bo, LIU Meichen, ZHOU Biying. Effect of excretory-secretory antigen TPx of Cysticercus cellulosae on activation of dendritic cells in piglets [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 286-293.
[4]	RAOWAN Tuolehong, ABUDUSALAMU Abulikemu, YANG Lingfei, CHEN Lu, LI Zhao, JIA Fang, SONG Tao. Effect evaluation and factor analysis of ultrasonic manifestations in the diagnosis of hepatic alveolar echinococcosis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 312-318.
[5]	KUI Yan, XUE Chuizhao, WANG Xu, LIU Baixue, WANG Ying, WANG Liying, YANG Shijie, HAN Shuai, WU Weiping, XIAO Ning. Progress of echinococcosis control in China, 2021 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 142-148.
[6]	MA Hui, CHONG Shigui, CHEN Gen, ZHANG Linghui, QIN Junmei, ZHAO Yumin. Research progress on the cellular signal pathways associated in alveolar echinococcosis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 223-227.
[7]	AN Xiu-qing, WANG Miao-miao, ZHOU Hong-qian, MENG Kai, CAI Jian-ping, LIU Guang-hui, A Ji-de, YANG Jing-yu. Research progress on microvascular density in hepatic alveolar echinococcosis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(6): 792-797.
[8]	ZHANG Ting-ting, DU Qiu-pei, GUO Xin-jian, ZHANG Ling-qiang, WANG Zhi-xin, CHANG Zheng-song, ZHAO Qian, WANG Hai-jiu, HOU Li-zhao. Research progress on vascular invasion of hepatic alveolar echinococcosis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(4): 516-523.
[9]	WU Liang-liang, YANG Ling-fei, SONG Tao. Ultrasound and pathological manifestations of lesions in SD rats with hepatic Echinococcus multilocularis infection established by different methods [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(4): 549-552.
[10]	ZHANG Ling-hui, CHEN Gen, CHONG Shi-gui, SHEN Hui, MA Hui, ZHAO Yu-min. Research progress on the immune regulation mechanism in alveolar echinococcosis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 109-113.
[11]	CHEN Sui-lin, GAO Yuan-li, GUO Shuai, FAN Yong-ling, LIU Tai-ping, XU Wen-yue. Effect and mechanism of high-dose clodronate liposomes treatment on Plasmodium yoelii growth in mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 28-35.
[12]	SHAO Han, LI Si-yuan, LI Jun. The affect of metformin on autophagy and apoptosis of Echinococcus multilocularis cysts and protoscoleces [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 43-49.
[13]	YANG Liu, HE Wei, WANG Qi, YU Wen-jie, ZHONG Bo, LIU Yang, XIAO Tong-guang, XIE Fei, YAO Ren-xin, HUANG Yan, LI Rui-rui, LIAO Sha, ZHANG Guang-jia, WANG Qian. The impact of reducing stray dog density on the prevalence of Echinococcus spp. in small mammals [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(2): 156-160.
[14]	YANG Hai-cheng, ZHANG Hong-wei, SHI Kang-jie, WEN Yu-peng, LIU Shi-wen, ZHANG Yong-guo, ZHU Zhi-qiang, ZHANG Shi-jie. Autocrine osteopontin promotes the growth and metastasis of Echinococcus multilocularis via the EGFR signaling pathway [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(2): 226-232.
[15]	XU Kai, WANG Hai-jiu, ZHANG Li, ZHANG Yao-gang, FAN Hai-ning, REN Li, REN Bin, WANG Zhi-xin. Research progress on the mechanisms underlying the impairment of host hepatocytes by Echinococcus multilocularis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(2): 256-259.