Morphological observation and phenotypic detection of dendritic cells from peripheral blood of patients with alveolar echinococcosis

Abstract

Abstract:

Objective To understand the morphological and phenotypic characteristics of dendritic cells (DCs) from peripheral blood of patients with alveolar echinococcosis. Methods Peripheral blood was collected from 10 patients with alveolar echinococcosis (AE), 10 healthy Han participants (HH) (n = 10) and 10 healthy Tibetan participants (TH), respectively. Monocytes were isolated, and cultured by adherent culture. The monocytes were induced into DCs with recombinant human granulocyte macrophage colony stimulating factor and recombinant human interleukin 4. DCs were collected on days 1, 3, 5 and 7 of culture and cell morphology was observed by inverted microscopy and scanning electron microscopy. The positive rate of cell surface markers was detected by flow cytometry on day 7. Data were analyzed with SPSS 22.0 software. Results On day 1 of in vitro induction, the majority of DCs in the 3 groups had a single round shape with clear boundary, bright cytoplasm, and a small volume, floating in the culture medium. On day 3 of induction, the three groups of DCs aggregated into semi-suspension and the cells enlarged with an irregular shape. On day 5 of induction, the three groups of DCs formed colonies, and most DCs had a rough surface. On day 7 of induction, the three groups of DCs grew into semi-suspension with a filamentous cell edges. Compared to the HH and TH groups, the number of DC colonies in group AE was smaller, and the irregular protuberances of DC were not obvious, displaying an atypical DC morphology. The positive expression rates of CD1a, CD80 and CD86 on the surface of DCs induced differentiation in AE group (12.73% ± 1.73%, 12.41% ± 2.83% and 16.34% ± 3.59%, respectively) were significantly lower than those in the HH and TH groups (P < 0.05), while those of HH group (18.40% ± 1.20%, 20.77% ± 3.40%, 31.78% ± 5.02%) and TH group(17.50% ± 1.44%, 23.75% ± 5.33%, 33.20% ± 2.47%) were not significantly different (P > 0.05). Conclusion The induced DCs in the AE group have an atypical morphology and a decreased level of cell surface markers, indicating the occurrence of maturation disorder.

Key words: Alveolar echinococcosis, Dendritic cell, Cell morphology, Immunophenotype

CLC Number:

R532.32

Yong FU, Ru MENG, Hai-fang XUE, Hai-ning FAN, Hai-feng NIU, Zi-jia ZHOU, Hong-bin WANG. Morphological observation and phenotypic detection of dendritic cells from peripheral blood of patients with alveolar echinococcosis[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(5): 474-477.

Figures/Tables 3

References 12

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实验分组Experimental group	DCs表面标志分子阳性表达率/% Positive expression rate of surface molecules on dendritic cells/%
实验分组Experimental group	CD1a	CD80	CD86
汉族健康志愿者Healthy Han volunteers	18.40 ± 1.20	20.77 ± 3.40	31.78 ± 5.02
藏族健康志愿者Healthy Tibetan volunteers	17.50 ± 1.44	23.75 ± 5.33	33.20 ± 2.47
多房棘球蚴病患者Alveolar echinococcosis patients	12.73 ± 1.73^a	12.41 ± 2.83^a	16.34 ± 3.59^a

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