Scanning electron microscopic and transmission electron microscopic observations of the tegument structure of adult Clonorchis sinensis

Abstract

Abstract:

To study the ultrastructural characteristics of Clonorchis sinensis, scanning electron microscopy (SEM) was performed to observe the body surface of adult C. sinensis obtained from an infected rat, and transmission electron microscopy (TEM) was used to observe the tegument structure. By SEM, we found a number of nodules connected by filaments on the body surface of the parasite, with unevenly-distributed different sizes of sense papillae protruding from the body surface. The TEM showed that the epidermis of the C. sinensis was composed of tegument, the muscle layer and the cell layer. The surface of the tegument was covered with numerous protrusions containing mitochondria, secretory granules and secretory vesicles. The outermost-layer protrusions showed a tendency to detach from the tegument. The matrix contained spherical and rod-shaped secretory granules, secretory vesicles and mitochondria. The muscular layer was distributed with circular and longitudinal muscles. In the cell body layer, cell nuclei were clearly seen and the cytoplasm contained two types of secretory bodies and secretory vesicles. The cell body layer and the tegument were connected by cytoplasmic bridges.

Key words: Clonorchis sinensis, Tegument, Scanning electron microscopy, Transmission electron microscopy

CLC Number:

R383.22

Yun-liang SHI, Xiao-ling WAN, Zhi-hua JIANG, Xiao-jing CHENG, Yi-chao YANG. Scanning electron microscopic and transmission electron microscopic observations of the tegument structure of adult Clonorchis sinensis[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(2): 184-186.

Figures/Tables 5

References 15

[1]	Srivatanakul P, Sriplung H, Deerasamee S.Epidemiology of liver cancer: an overview[J]. Asian Pac J Cancer Prev, 2004, 5(2): 118-125.
[2]	Chen J, Xu MJ, Zhou DH, et al. Canine and feline parasitic zoonoses in China[J]. Parasit Vectors, 2012, 5: 152.
[3]	Choi D, Lim JH, Lee KT, et al. Cholangiocarcinoma and Clonorchis sinensis infection: a case-control study in Korea[J]. J Hepatol, 2006, 44(6): 1066-1073.
[4]	Bouvard V, Baan R, Straif K, et al. A review of human carcinogens-Part B: biological agents[J]. Lancet Oncol, 2009, 10(4): 321-322.
[5]	Dalton JP, Skelly P, Halton DW.Role of the tegument and gut in nutrient uptake by parasitic platyhelminths[J]. Can J Zool, 2004, 82(2): 211-232.
[6]	McLaren DJ, Terry RJ. The protective role of acquired host antigens during schistosome maturation[J]. Parasite Immunol, 1982, 4(2): 129-148.
[7]	Camacho M, Agnew A.Schistosoma: rate of glucose import is altered by acetylcholine interaction with tegumental acetylcholine receptors and acetylcholinesterase[J]. Exp Parasitol, 1995, 81(4): 584-591.
[8]	Skelly PJ, Alan Wilson R.Making sense of the schistosome surface[J]. Adv Parasitol, 2006, 63: 185-284.
[9]	Fonseca CT, Braz FCG, Carvalho AC, et al. Schistosoma tegument proteins in vaccine and diagnosis development: an update[J]. J Parasitol Res, 2012, 2012: 541268.
[10]	Sobhon P, Anantavara S, Dangprasert T, et al. Fasciola gigantica: studies of the tegument as a basis for the developments of immunodiagnosis and vaccine[J]. Southeast Asian J Trop Med Public Health, 1998, 29(2): 387-400.
[11]	刘铁聪, 王翠霞, 邓立军, 等. 肝片形吸虫成虫的扫描电镜观察[J]. 畜牧兽医学报, 1986, 17(4): 268-270.
[12]	何毅勋, 谢觅. 日本血吸虫尾蚴体表结构的扫描及透射电镜观察[J]. 武夷科学, 1992, 9(1): 153-158.
[13]	程由注, 许国防, 杨文川, 等. 东方次睾吸虫成虫体被扫描电镜研究[J]. 中国人兽共患病杂志, 2005, 21(10): 875-879.
[14]	Pearce EJ, Basch PF, Sher A.Evidence that the reduced surface antigenicity of developing Schistosoma mansoni schistosomula is due to antigen shedding rather than host molecule acquisition[J]. Parasite Immunol, 1986, 8(1): 79-94.
[15]	Schmidt J.Glycans with N-acetyllactosamine type 2-like residues covering adult Schistosoma mansoni, and glycomimesis as a putative mechanism of immune evasion[J]. Parasitology, 1995, 111(Pt 3): 325-336.