Composition of anopheline larvae in the China-Myanmar border region in Yingjiang County of Yunnan Province

Abstract

Abstract:

Objective To understand the species composition of anopheline larvae in the China-Myanmar border region in Yingjiang County, Yunnan Province of China. Methods The Kayahe Village in Nabang Town and Huqueba Village in Pingyuan Town were selected for determining the breeding-place of anophelines. The composition of anopheline larvae was investigated once a month from June to October, 2016, in potential habitats of slack water, puddle, pond, moor, and paddy. The values of pH and area, and the numbers of first-, second-, third-, and fourth-instar larvae as well as pupae of mosquitoes were recorded in each habitat. The species of late third-instar and fourth-instar anopheline mosquito larvae were identified under microscope using commonly accepted guidelines. Pupae were morphologically identified after the emergence. Species composition of anopheline larvae was analyzed using six indices, including density(Ds), Simpson diversity index(D), Shannon diversity index(H), Berger-Parker dominance index(d), and Shannon evenness index (E). The Morisita-Horn similarity index (C) was employed to calculate the similarity of species composition between the two towns. Results A total of 394 larvae samples were collected in 91 specimen collection sites, comprising 313 samples (268 Anopheles and 45 Culex) from Nabang Town and 81 samples(55 Anopheles and 26 Culex) from Pingyuan Town. The Anopheles in Nabang Town were predominated by An. sinensis(183, 68.28%), followed by An. peditaeniatus(33, 13.31%) and An. minimus (25, 9.33%), and those in Pingyuan Town were mainly An. sinensis(27, 49.09%), An. peditaeniatus (24, 43.64%), and An. vagus(2, 3.64%). In Yingjiang County, the Ds of Anopheles was 0.33 per dip, with D, H, d and E being 0.54, 1.16, 0.65 and 0.50, respectively. The values of N, H, and d were relatively higher in Nabang Town, with an E value approaching 0.5, albeit with a slightly lower D value. The values of Ds, N, and H in Nabang Town were higher than those in Pingyuan Town in each month. Puddles were rich in various species of anopheline larvae, with a smaller d and a higher N. D and H were highest in this habitat, and the value of E was most approaching 0.5. Large water habitats (with an area > 100 m²) had more abundant species, with higher D and H values, smaller d, and an E value more approaching 0.5. Habitats with slightly acidic water(pH < 7) had a complicated vector composition. Conclusion There are abundant species of anopheline larvae in habitats with a large area and in puddles with slightly acidic water in the China-Myanmar region in Yunnan Province, with a complicated structure of composition. There is also a high similarity in vectors between the towns.

Key words: Anopheles, Larva, Composition structure, Diversity, Similarity

CLC Number:

R384.111

CHEN Tian-mu, ZHOU Hong-ning, LUO Chun-hai, ZENG Xu-can, GUO Xiang-rui, LIN Zu-rui, TU Hong, WANG Xue-zhong, ZHANG Shao-sen, ZHOU Shui-sen. Composition of anopheline larvae in the China-Myanmar border region in Yingjiang County of Yunnan Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(2): 112-118.

Figures/Tables 4

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References 30

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种类Specie	那邦镇Nabang Town		平原镇Pingyuan Town		合计Total
种类Specie	幼虫数No. larve	构成/% Composition/%	幼虫数No. larve	构成/% Composition/%	幼虫数No. larve	构成/% Composition/%
中华按蚊An. sinensis	183	68.28	27	49.09	210	65.02
带足按蚊An. peditaeniatus	25	9.33	24	43.64	49	15.17
微小按蚊An. minimus	33	12.31	1	1.82	34	10.53
库态按蚊An. culicifacies	15	5.60	0	0	15	4.64
迷走按蚊An. vagus	5	1.87	2	3.64	7	2.17
孟加拉按蚊 An. bengalensis	2	0.75	0	0	2	0.62
美彩按蚊An. splendidus	1	0.37	1	1.82	2	0.62
合计Total	264	100	55	100	319	100

乡（镇）Town	密度/只·勺^-1 Density/Larva·dip^-1	N	D	H	d	E	C
那邦Nabang	0.37	10	0.51	1.11	0.68	0.48
平原 Pingyuan	0.22	5	0.57	0.98	0.49	0.61
合计Total	0.33	10	0.54	1.16	0.65	0.50	0.82

乡镇Town	水体类型Habitats	幼蚊种类Species	N	D	H	d	E
那邦Nabang	缓流Slack water	中华按蚊An. sinensis	3	0.12	0.28	0.94	0.26
		带足按蚊An. peditaeniatus
		库态按蚊An. culicifacies
	积水Puddle	微小按蚊An. minimus	10	0.61	1.32	0.58	0.57
		中华按蚊An. sinensis
		棋斑按蚊An. tessellatus
		迷糊按蚊An. vagus
		环斑按蚊An. annularis
		孟加拉按蚊An. bengalensis
		美彩按蚊An. splendidus
		须喙按蚊An. barbirostris
		带足按蚊An. peditaeniatus
		库态按蚊An. culicifacies
	水潭Pond	中华按蚊An. sinensis	2	0.32	0.50	0.80	0.72
		带足按蚊An. peditaeniatus
	小型沼泽Moor	中华按蚊An. sinensis	2	0.10	0.20	0.95	0.29
		带足按蚊An. peditaeniatus
平原Pingyuan	水田Paddy	中华按蚊An. sinensis	2	0.46	0.65	0.65	0.94
		带足按蚊An. peditaeniatus
	积水Puddle	微小按蚊An. minimus	5	0.59	1.06	0.47	0.66
		中华按蚊An. sinensis
		迷糊按蚊An. vagus
		美彩按蚊An. splendidus
		带足按蚊An. peditaeniatus