Dot Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibody to Blastocystis hominis in Humans

›› 2007, Vol. 25 ›› Issue (3): 23-258.

• 研究简报 • Previous Articles Next Articles

Dot Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibody to Blastocystis hominis in Humans

SU Shui-lian;YAN Yi-ming;LIAO Hua;CHEN Gui-feng;ZHANG Rui-qi;XIE Qiong-jun;LE Xiao;HU Ya-qiong;ZENG Xue-ying;LAN Hai-ying;XIE Rui-lian;Huang Zhen

Gannan Medical College，Ganzhou 341000，China

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30
Contact: YAN Yi-ming

Abstract

Abstract: 【Abstract】 Serum and stool samples were collected from 322 undergraduate students in medical school. Using stool in vitro cultivation as golden standard， 178 cases were found Blastocystis hominis positive and 144 were negative. Dot-ELISA was used to examine the serum samples with a sensitivity of 92.1% （164/178） and specificity of 97.1% （141/144）. This revealed that dot-ELISA can be used for antibody detection against Blastocystis hominis.

Key words: Blastocystis hominis, Dot-ELISA, Antibody, In vitro cultivation

SUShui-lian;YANYi-ming;LIAOHua;CHENGui-feng;ZHANGRui-qi;XIEQiong-jun;LEXiao;HUYa-qiong;ZENGXue-ying;LANHai-ying;XIERui-lian;HuangZhen. Dot Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibody to Blastocystis hominis in Humans[J]. , 2007, 25(3): 23-258.

[1]	ZHU Mingchao, ZHU Ya, ZHAO Jianzhong, YUAN Huizhen. Analysis of Blastocystis hominis infection in long-term hospitalized patients in Tianmen City [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 121-124.
[2]	ZOU Wei-hao, WU Wei-ling, LIAO Yuan-peng, CHEN Min, PENG Hong-juan. Preparation and application of monoclonal antibody against Toxoplasma gondii bradyzoite antigen 1 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 587-593.
[3]	ZHU Ming-chao, ZHU Ya. HIV co-infection with Blastocystis hominis and Giardia lamblia in a patient [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 315-318.
[4]	QIAN Ke, HU Zhu-hua, WU Xiu-ping, PENG Guo-hua, YU Ying-fang, CHU Yan-hong, ZHOU Xian-feng, XU Dan, TENG Xue-jiao, LI Hui, TIAN Li-guang. Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(1): 55-60.
[5]	LI Qin-cui, CHEN Feng, GU Wei, YANG Lei. Clinical features of 65 cases of fascioliasis in Yunnan Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(4): 521-523.
[6]	Yin-long LI, Zhang-ke YU, You-xing LI, Ding-hua AI, Ping ZHANG, Zhao-jun LI, Dan-dan LIN, Jing XU. Case management for those with positive anti-Schistosoma antibody in Poyang Lake of Jiangxi Province area after transmission control [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(6): 665-669.
[7]	Ya-ling ZHOU, Liang WU, Qing YIN, Yao WU, Lei HE, Zhi-qing ZOU, Xiao-yue DAI, Wen XIA. Infections of Clostridium difficile, Blastocystis hominis and Cryptosporidium spp. in patients with chronic diarrhea [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(6): 723-726.
[8]	Xue-mei ZENG, Zhi-hua JIANG, Ji-qing SHEN, Qing-li YANG. Detection and analysis of fecal egg and serum IgG antibody in 146 suspected cases of Clonorchis sinensis infection in Guangxi [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(6): 730-732.
[9]	Rui YANG, Yi-ding XIONG, Shan-shan GUO, Ye LIAO, Qiang XIA, Jin-jin GUO, Lian-feng DU. Inhibitory effect of anti-profilin antibody on the proliferation of Toxoplasma gondii in mouse embryonic fibroblasts [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(4): 437-443.
[10]	Zheng-hu ZHAO, Sheng-nan QIAN, Jing-yi WAN, Zi-ru TANG, Shuang SHEN. Preparation of polyclonal antibody against-triosephosphate isomerase of Toxoplasma gondii and its application to detect T. gondii infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(3): 311-316.
[11]	Jing LIU, Jyh-wei SHIN, Pei PEI, Xiao-yin FU, De-jun LIAO, Yun-yu LU, Sa XUE, Chen-chieh LIAO, Deng-yu LIU. A modified method to infect Blastocystis hominis in rats and the pathological changes after infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(2): 161-167.
[12]	Xue-jiao TENG, Yan-hong CHU, Cheng-cheng ZHAI, Ying-fang YU, Yu-chun CAI, Shao-hong CHEN, Lin AI, Li-guang TIAN, Jia-xu CHEN. The epidemiological characteristics and influencing factors for Blastocystis hominis infection among human immunodeficiency virus seropositive individuals in Tengchong of Yunnan Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(2): 129-134.
[13]	Jyh-wei SHIN, Chen-Chieh LIAO, De-jun LIAO, Xu-ning SUN, Xiao-yin FU, Deng-yu LIU. Modification of axenic culture medium and morphological observation of Blastocystis hominis in vitro [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(6): 623-625.
[14]	Xiao LIU, Meng-meng LI, Feng QIAN. Injection of anti-TLR9 antibody cannot efficiently block TLR9 activities in mice: a brief discussion about TLR9 location in cells [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(5): 485-488.
[15]	ZHANG Hao1*, MA Wen-jing2, HE Shao-heng3. Production and identification of monoclonal antibodies against cockroach allergen Bla g 2 [J]. , 2017, 35(4): 10-362-365.

Dot Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibody to Blastocystis hominis in Humans

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments