Infections of Clostridium difficile, Blastocystis hominis and Cryptosporidium spp. in patients with chronic diarrhea

doi:10.12140/j.issn.1000-7423.2019.06.020

Abstract

Abstract:

Ninety-six stool samples were collected from patients with chronic diarrhea at affiliated hospital of Jiangsu University (inpatients and outpatients) in 2018. Each stool sample was smeared for microscopic examination. At the same time fecal DNA was extracted. The genes for 16S rDNA, toxin A (tcdA), toxin B (tcdB), binary toxin (cdtA and cdtB) of Clostridium difficile were detected by multiplex PCR. The SSU rRNA gene of Blastocystis hominis was detected by conventional PCR and the SSU rRNA gene of Cryptosporidium spp. was detected by nest-PCR. C. difficile 16S rDNA was detected in 64 out of 96 patients with chronic diarrhea with a positive rate of 66.7%. C. difficile TcdA gene was detected in 24 cases and tcdB gene in 18 cases of all C. difficile positive samples. There was no sample with only tcdB. No cdtA and cdtB genes were detected in any C. difficile positive sample. The rate of toxigenic strain was 37.5%. Microscopic examination detected 16 B. hominis infected samples with detection rate of 16.7%. PCR results showed that 21 samples B. hominis SSU rRNA gene was amplified in 96 stool samples with a detection rate of 21.9%. All microscopic positive samples were positive in PCR detection. No mixed infection of C. difficile and B. hominis was detected. The Cryptosporidium spp. SSU rRNA gene was not detected in all stool samples. The results showed that C. difficile and B. hominis were the main organisms detected in patients with chronic diarrhea in Nanjing area.

Key words: Chronic diarrhea, Clostridium difficile, Blastocystis hominis, Cryptosporidium spp.

CLC Number:

R532.23

Ya-ling ZHOU, Liang WU, Qing YIN, Yao WU, Lei HE, Zhi-qing ZOU, Xiao-yue DAI, Wen XIA. Infections of Clostridium difficile, Blastocystis hominis and Cryptosporidium spp. in patients with chronic diarrhea[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(6): 723-726.

Figures/Tables 4

References 25

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基因	引物序列（5′→3′）	预计产物大小/bp
艰难梭菌 tcdA	GCATGATAAGGCAACTTCAGTGGTA	629
	AGTTCCTCCTGCTCCATCAAATG
tcdB	CCAAARTGGAGTGTTACAAACAGGTG
	GCATTTCTCCATTCTCAGCAAAGTA	410
	GCATTTCTCCGTTTTCAGCAAAGTA
cdtA	GGGAAGCACTATATTAAAGCAGAAGC
	GGGAAACATTATATTAAAGCAGAAGC	221
	CTGGGTTAGGATTATTTACTGGACCA
cdtB	TTGACCCAAAGTTGATGTCTGATTG	262
	CGGATCTCTTGCTTCAGTCTTTATAG
16S rDNA	GGAGGCAGCAGTGGGGAATA	1 062
	TGACGGGCGGTGTGTACAAG
人芽囊原虫 SSU rRNA	GAGCTTTTTAAGTCCAACAACG	600
	ATCTGGTTCATGGTTGCGAGT
隐孢子虫 SSUrRNA 第1轮	TTCTAGAGCTAATACATGCG	-
	CCCATTTCCTTCGAAACAGGA
第2轮	GGAAGGGTTGTATTTATTAGATAAAG	850
	AAGGAGTAAGGAACAACCTCCA