Modulation of Immune Response to Plasmodium falciparum Apical Membrane Antigen 1 DNA Vaccine by Cytokine Plasmids

Abstract

Abstract: 　Objective To explore the effect of cytokine encoding plasmids on DNA immunization in mice. Methods Prototype DNA vaccine VR1020/E which contain Plasmodium falciparum apical membrane antigen 1 (AMA1) ectodomain was constructed, and eukaryotic expression vectors pcDNA3/GM-CSF, pcDNA3.1(-)/IL-4, pIL-12 and pGM-CSF/pTPA-E were also built. BALB/c mice were immunized with VR1020/E alone or with VR1020/E plus different cytokine plasmids. Serum IgG and its subtype were determined by ELISA and in vitro splenocyte proliferation assay was done. Results GM-CSF, IL-4 and IL-12 encoding plasmids all promoted mice immune response to VR1020/E, the antibody level increased 7 to 10 times and splenocyte proliferation was enhanced too. Plasmid pcDNA3/GM-CSF induced much more IgG1 whereas plasmid pIL-12 induced much more IgG2a. Conclusion Cytokine encoding plasmids might be used as adjuvant in AMA1 DNA immunization.

Key words: Plasmodium falciparum, Apical membrane antigen 1, DNA vaccine, Cytokines, Adjuvant, Immunologic

LIXun;MIAOJun;LEIJun-chuan;XUECai-fang;WANGXian-feng;LIUZhong-xiang;LIShu-mei. Modulation of Immune Response to Plasmodium falciparum Apical Membrane Antigen 1 DNA Vaccine by Cytokine Plasmids[J]. , 2004, 22(3): 3-143.

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Modulation of Immune Response to Plasmodium falciparum Apical Membrane Antigen 1 DNA Vaccine by Cytokine Plasmids

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