Screening of Mimic Epitopes of Trichinella spiralis Antigen from Phage 12-mer Peptide Library

Abstract

Abstract: 　Objective To screen special mimic epitopes of Trichinella spiralis antigen from peptide library for exploring new diagnostic antigens. Methods Ts-IgG purified from serum of trichinosis patients was used to screen the phage 12-mer peptide library for 5 rounds. 24 clones were picked out randomly to detect the immunoactivity. The sensitivity and specificity of the 6 clones (T1-T6) whose A values were higher than others were tested by ELISA. Results The sensitivity of the clones T1-T6 was the same with larval antigen of Trichinella spiralis (TsA) (positive rate: 100%, P>0.05), and there was no difference in specificity between T1-T6 and TsA (negative rate: 0-40%, P>0.05); T3 and T6 did not react with sera from patients of paragonimiasis, showing higher specificity than TsA(P<0.05); T6 did not react with sera from patients of schistosomiasis, also showing higher specificity than TsA(P<0.05). Conclusion The mimic antigenic epitopes of Trichinella spiralis have been successfully obtained by screening phage 12-mer peptide library.

Key words: Trichinella spiralis, trichinosis, phages, peptide library

PANHong;JIANGChang-fu;LITian-qun;LEIJia-hui;SHIHong-bo;WEILan-ying. Screening of Mimic Epitopes of Trichinella spiralis Antigen from Phage 12-mer Peptide Library[J]. , 2004, 22(2): 11-105.

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Screening of Mimic Epitopes of Trichinella spiralis Antigen from Phage 12-mer Peptide Library

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