EXPERIMENTAL STUDY ON THE EFFECT OF ARTEMETHER AGAINST TOXOPLASMA 　GONDII IN VITRO

Abstract

Abstract: AIM:To study the activities of artemether(Art) against the tachyzoite of Toxoplasma gondii in
vitro. METHODS:The method of cell culture of rat kidney was used for investigation the effects of art
(concentration0.0 1-100 μg/ml) on the invasion and proliferation of T.gondii. Acetylspiramycin was used as control. RESULTS:The minimum effective concentration of Artagainst T.gondii was 0.1 μg/ml. When the concentration of Art was 4μg/ml, most of the Toxoplasma organisms appeared deformed and distorted after 48h with vacuoles and granules in its cytoplasm under light microscope. The anti Toxoplasma effect of acetylspiramycin was inferior to that of Art at the same concentration. CONCLUSION: Art could inhibit the invasion and multiplication of T.gondii tachyzoites and kill them in vitro. The toxoplasm acidal effect of Art was proved to be superior to that of acetylspiramycin.

Key words: Artemether, Toxoplasma gondii, cell culture, drug effects

LiuYang;ZhangYonghao;LiZhe;YaoXiangmin. EXPERIMENTAL STUDY ON THE EFFECT OF ARTEMETHER AGAINST TOXOPLASMA 　GONDII IN VITRO[J]. , 1997, 15(6): 366-369.

[1]	XUE Yushan, LIN Ping, CHENG Xunjia, FENG Meng. Damage caused by chronic infection of Toxoplasma gondii on the host central nervous system and its mechanism [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 527-531.
[2]	JIANG Wenjing, MENG Yali, ZHAO Lina, WANG Chunmiao, ZHANG Xiaolei. Immunoprotection of nuclei acid vaccine dual-targeting rhoptry protein 18 and surface antigen 30 of Toxoplasma gondii in mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 532-538.
[3]	ZHAO Ziqi, LV Fangli. Study on the inhibitory effect of artemether liposome on Toxoplasma gondii proliferation in vitro [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 446-451.
[4]	ZHANG Chi, CHEN Jiating, XIN Zixuan, YANG Lili, YANG Zihan, PENG Hongjuan. Transcriptome analysis of mice brain chronically infected with Toxoplasma gondii and validation of the kynurenine pathway associated with depression [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 270-278.
[5]	OU Yangran, LIU Xingzhuo, HUANG Shiguang, LYU Fangli. Effect of locking galectin-receptor interaction on the immunopathology of small intestine of Toxoplasma gondii-infected mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 279-285.
[6]	DU Juan, LI Jia, WU Di, YU Qi, ZHANG Wei, BAI Runian, GUO Junlin, LIU Qingbin, LEI Qili, GU Chuanhui, WANG Meng, ZHAO Haojun. Seroepidemiological survey of Toxoplasma gondii infection in dogs and cats in Beijing 2022 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 389-392.
[7]	LI Jia-ming, WANG Yi-xuan, YANG Ning-ai, MA Hui-hui, LAN Min, LIU Chun-lan, ZHAO Zhi-jun. Effects of ROP16 protein of Toxoplasma gondii on polarization and apoptosis of MH-S cells and their related mechanisms [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 579-586.
[8]	ZOU Wei-hao, WU Wei-ling, LIAO Yuan-peng, CHEN Min, PENG Hong-juan. Preparation and application of monoclonal antibody against Toxoplasma gondii bradyzoite antigen 1 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 587-593.
[9]	DAI Li-sha, ZHANG Li-xin, YIN Kun. Research advances in Toxoplasma gondii induced host mental-behavioural disorders [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 642-646.
[10]	WANG Jie, WEN Hong-yang, CHEN Ying, AN Ran, LUO Qing-li, SHEN Ji-long, DU Jian. Construction and identification of macrophage migration inhibitory factor gene knockout strain of Toxoplasma gondii [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 349-354.
[11]	WANG Zhen-xun, XIONG Si-si, SUN Xia-hui, WANG Yong-liang, PAN Ge, HE Shen-yi, CONG Hua. Differential expression and action mechanism of lncRNA102796 in the brain of mice with chronic infection of Toxoplasma gondii [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(2): 187-193.
[12]	JIANG Feng, CHEN Run, DU Ning-ning, ZHU Meng-yi, ZHONG Hao, CHEN Hui, XI Xu-xia, ZHAN Xiao-dong, LI Chao-pin. Investigation of Toxoplasma gondii infection in pet dogs and cats in Wuhu City [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 124-126.
[13]	LU Fei, ZHUO Xun-hui, LU Shao-hong. Research progress on the interaction between host cell autophagy and apicomplexa protozoa infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(6): 826-831.
[14]	ZHANG Xiao-han, FENG Ying, CHEN Ran, SANG Xiao-yu, YANG Na. Advances in research of structure, function and regulatory mechanism of Toxoplasma gondii conoid [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(6): 832-835.
[15]	WANG Long-jiang, LI Jin, YIN Kun, XU Chao, LIU Gong-zhen, HUANG Bing-cheng, WEI Qing-kuan, SUN Hui. Comparative analysis of transcriptomes in Toxoplasma gondii before and after invasion in human foreskin fibroblasts [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(4): 480-486.