›› 1997, Vol. 15 ›› Issue (6): 370-372.

• 论著 • Previous Articles     Next Articles

CONSTRUCTION OF THE c DNA LIBRARY OF LEISHMANIA DONOVANI

Jing Baoqian1; Hu Xiaosu2; Chen Jianping2; L iu Hongbin2   

  1. 1 Department of Microbiology, North Sichuan Medical College, Nanchong 637000 2 Laboratory of Parasitology, West China University of Medical Sciences, Chengdu 610044
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

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Abstract: AIM:To isolate indivadual gene products and to detect the gene characterization of L.donovani. METHODS:cDNA was synthesized with the mRNA temple isolated from the L.donovani mastigotes in vitro and the cDNA
library was constructed with the λgt11 DNA arm as the vecters. RESUL TS:2×106 recombinants were produced and the
insert proportion was48%. 2×104 recombinants infected to E.coli Y1090r- were screened with a rabbit serum that vaccinated with L.donovani mastigotes as the probe, 3 positive expressing clones were detected, the molecular weight of the expressing peptides were 136 kDa, 160 kDa and 148 kDa, respectively. CONCLUSION: An expressing cDNA library of L.donovani was constructed, and its insert proportion and expressing rate were high.

Key words: Leishmania donovani, cDNA library, immune screening