Prevalence of Paragonimus metacercariae infection and genotyping of Paragonimus metacercariae in freshwater crabs from Jianning County, Fujian Province

doi:10.12140/j.issn.1000-7423.2025.03.020

Abstract

Abstract:

To investigate the prevalence of Paragonimus metacercariae infections and genotyping of Paragonimus metacercariae in freshwater crabs from Jianning County, Fujian Province, freshwater crabs were collected from mountain streams and ditches near residential areas along the Chuxi River system in Jianning County, Fujian Province, and Paragonimus metacercariae were collected by twice filtering through the sieves for morphological characterization. Genomic DNA was extracted from Paragonimus metacercariae, and the ribosomal internal transcribed spacer 2 (ITS2) gene sequence was amplified using PCR assay. Following sequencing of the PCR amplification product, the gene sequence was uploaded to the GenBank database, and gene sequence alignment was performed using the BLAST tool. A phylogenetic tree was built using the neighbor-joining method with the MEGA software with Fasciola hepatica as an outgroup. A total of 71 freshwater crabs were captured, and there were 60 crabs infected with Paragonimus metacercariae, with an 84.51% prevalence rate of infections. A total of 782 Paragonimus metacercariae were identified, and the intensity of infections was 13 metacercariae per crab. The PCR amplification product were approximately 500 bp in length. The ITS2 gene sequence from this study (GenBank accession No.: PQ510326.1) was 99.13%, 98.79%, and 99.18% identical to P. westermani isolates from Jilin Province (GenBank accession No.: AB713404.1), Shanghai Municipality (GenBank accession No.: KC417492.1), and Zhejiang Province (GenBank accession No.:JQ354935.1), respectively. Phylogenetic analysis revealed that the two gene sequences obtained from this study were clustered into the same clade with sequences of P. westermani isolates from Jilin Province (GenBank accession No.:AB713404.1), Shanghai Municipality (GenBank accession No.: KC417492.1), and Zhejiang Province (GenBank accession No.: JQ354935.1) at a genetic distance of 0, and they exhibited genetic distances of 0.079 and 0.075 P. skrjabini isolates (GenBank accessions No.: AB325516.1 and AB703444.1) and 0.078, and 0.078 from P. miyazakii isolates (GenBank accessions No.: AB713405.1 and AY618741.1), with distinct clade separation. The gene sequences of P. westermani isolates from this study share high identities to those of P. westermani isolates from other provinces, suggesting that these P. westermani isolates may serve as a reference isolate of P. westermani in Fujian Province.

Key words: Paragonimus, ITS2, Genotyping, Phylogeny, Fujian Province

CLC Number:

R532.221

GAO Lanlin, XIE Hanguo, JIANG Dianwei, CAI Wuwei, ZHENG Dan. Prevalence of Paragonimus metacercariae infection and genotyping of Paragonimus metacercariae in freshwater crabs from Jianning County, Fujian Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(3): 436-439.

Figures/Tables 3

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