关键词: 恶性疟原虫, 富组氨酸蛋白2, 疫苗, 免疫应答

Abstract: 　Objective To identify the immune characteristics of different vaccine prototypes based on HRP2 and to provide experimental evidence for developing P f. blood stage vaccines.Methods BALB/c mice were immunized with recombinant protein TP HRP2 or eukaryotic expression plasmid pcDNA3 1(-)/HRP2. The kinetics and specificities of antibody responses were analyzed. The proliferation tests of spleen cells were done, and P f. growth inhibition assays were done with immune sera.Results The mice immunized with TP HRP2 in Freund′s adjuvant produced high level and high specificity antibody response. The antibodies appeared rapidly and lasted for a longer time. Cellular responses were induced simultaneously, and the immune sera could inhibit the development of parasite in IRBCs. The mice immunized with pcDNA3 1(-)/HRP2 produced middle level antibody response which had some specificity,however,the induction of antibodies required repeated inoculation and a longer duration. Immune cells were well primed and the memorial immune response was obvious but the immune sera had no effect on the growth of P f. in vitro .Conclusion Both the recombinant protein and plasmid DNA based on HRP2 have different immune characteristics in mice. HRP2 recombinant protein has the potential in practical application.

Key words: Plasmodium falciparum, histidine rich protein 2, vaccine, immune response