关键词: 融合蛋白, 恶性疟原虫, 斑点ELISA

Abstract: The feasibility of using fusion protein Dot-ELISA to detect the antibodies to Plas-modium falciparum was presented. Fusion protein expressed in the P. falciparum genomic DNA expression library with λgtll. was used as the antigen to coat the solid support nitrocellulose strips. Horseradish peroxidase conjugated with goat antihuman IgG was used as the second antibody. The enzyme activity was read by naked eyes according to the dot colour. The results by Dot-ELISA correlated well with those by IFA. The study indicated that this method was specific, sensitive, simple, and replicable. It would be valuable for further studying the usefulness of fusion protein in the immuncdiagnosis of malaria.