关键词: 快速染色法, 表面活性剂, 疟原虫镜检, 非离子型, 血膜, 寄生虫学, 恶性疟原虫, 乳化剂OP, 染色程序, 染色方法

Abstract: Seven non-ionic surface active agents have been used in place of buffer solution pH 6.8 in Field Stain and the staining methods have been improved. The stains containing these non-ionic surface active agents can be prepared for immediate use due to their solubility. The stains kept in different flexible cans or ampoules are particularly suitable for the application in field research or survey. This staining method for thick films with complete hemolysis is highly applicable in field survey and clinical diagnosis of malaria. Both Schuffner's or Maurer's dots are visible in red cells infected with Plasmodium vivax or P. falciparum in thin smears. When 10% Giemsa solution prepared with 1% non-ionic surface active agent is used, the time for staining smears needs only 3 minutes.