Table of Content

28 February 1998, Volume 16 Issue 1

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论著

FIELD TRIALS ON THE EFFICACY OF ALBENDAZOLE COMPOSITE AGAINST INTESTINAL NEMATODIASIS

ZhangDehong;ZhangXiaorong;TangZhongjiuChenChuang;ChenYawei;YangWenjinJinMingan;HuangChengfang;YangGuangjian;LongYunkun

1998, 16(1):  1-5. 

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AIM: To study the anthelmintic effect of albendazole composite (containing 67 mg of albendazole and 83 3 mg base of pyrantel pamoate per tablet). METHODS: A randomized controlled study was carried out to compare the efficacy of a single dose of 3 or 2 tablets of albendazole composite versus a single dose of 400 mg of albendazole or 10 mg base/kg of pyrantel pamoate for treatment of intestinal nematodiasis including 1 864 cases infected with hookworm, 1 568 cases infected with Ascaris, 1 785 cases in...

RESEARCHES ON IMMUNOGENICITY IDENTIFICATION OF RECOMBINANT Sj 22.6(r Sj 22.6) kDa ANTIGEN GENE OF SCHISTOSOMA JAPONICUM 

angGuiyun;ZhangZhaosong;ChenShuzhen;ShenYiping;WuHaiwei;SuChuan;WuGuanling

1998, 16(1):  2-10. 

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AIM:To identify the immunogenicity and the potentiality of r Sj 22.6 as a candidate vaccine antigen for schistosomiasis japonica. METHODS:r Sj 22.6 was tested by Western blot with specific antibodies to identify its immunogenicity.The r Sj 22.6 protein was isolated and purified by SDS PAGE and injected twice into rabbits to raise antir Sj 22.6 serum that was tested by Western blot to recognize the native protein of adult S.japonicum and SEA.C57 mice were immunized with r Sj 2...

STUDY ON DETECTION OF MALARIA PARASITE DNA BY PCR ELISA *

ZhangLongxing**;TangLinhua;FengXiaoping;WangJujun

1998, 16(1):  3-15. 

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AIM: To present a new malaria diagnostic method based on detection of malaria parasite DNA by PCR ELISA. METHODS: According to the conserved sequence of Plasmodium SSUrRNA genes reported, a pair of primers in which one primer was biotinylated and another was unbiotinylated, suitable for DNA amplification of both falciparum and vivax malaria parasites were designed and synthesized. After denaturation and washing, the incoporated biotinylated product with avidin coated on plates previously was hyb...


ESTABLISHMENT OF HYMENOLEPIS DIMINUTA ANIMAL MODEL AND MORPHOLOGY OF CYSTICERCOID

YangWeiping;ShenYiping;ShaoJingou;XiangXiaoren;XuJuling

1998, 16(1):  4-20. 

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AIM:To observe the morphological changes in the process of the development of Hymenolepis diminuta. METHODS:The life cycle of Hymenolepis diminuta was established between Rattus domesticus albus and Triboliun castaneum .The morphology of cysticercoid were observed microscopically, and the ultrastructure of the body surface of cysticercoid were observed by scanning electron microscopy.RESULTS:Three phases including the mature stage,the bliste...

GENETIC ANALYSIS OF DELTAMETHRIN RESISTANCE IN CULEX PIPIENS PALLENS

ZhuChangliang;LiYulan;SunLixin;LiJianmin;GaoXiaohong;YeBinghui

1998, 16(1):  5-24. 

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AIM: To analyse the genetic character of deltamethrin resistant laboratory strain (resistant factor being 518) of Cx.pipiens pallens. METHODS: Genetic studies were made of deltamethrin susceptible and resistant strains of Cx.pipiens pallens with LC P curve contructed from the response of fourth instar larvae to deltamethrin treatment, and the resistance mechanisms were analysed with synergetic tests in PB, DEF and TPP. RESULTS: The resistant factors of F1 generation from cross and reciprocal ...


EFFECT OF ARTEMETHER ON HEXOKINASE, GLUCOSE PHOSPHATE ISOMERASE AND PHOSPHOFRUCTOKINASE OF SCHISTOSOMA JAPONICUM HARBORED IN MICE *

XiaoShuhua;YouJiqing;GuoHuifang;JiaoPeiying;MeiJingyan;YaoMinyi;FengZheng

1998, 16(1):  6-28. 

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AIM:To study the effect of artemether(Art) on hexokinase(HK),glucose phosphate isomerase(GPI)and phosphofructokinase(PFK)of Schistosoma japonicum . METHODS:Mice infected with schistosome cercariae for 4-5 wk were treated ig with Art 100 or 300 mg·kg -1 and killed 24 h or 48 h after medication for collection of schistosomes.The activities of HK,GPI and PFK of the worms were determined by measuring the formation of NADPH or consumption of NAD...


ESTIMATION OF VECTOR PREVALENCE BY USING POOL SAMPLING

GuWeidong

1998, 16(1):  7-33. 

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AIM:To investigate theoretically the feasibility of the pool sampling method in estimating the vector prevalence.METHODS:The probability distribution of infected individuals in a given pool was analyzed using binomial distribution,based upon the upper limit of predicted vector infectivity.The maximal pool size was determined in case that the probability of only one infected individual within this positive pool is larger than a predetermined significa...


COMPARATIVE OBSERVATION ON THE EFFICACY OF SEVERAL TEST/PROBE SYSTEMS FOR DETECTING SCHISTOSOME CIRCULATING ANTIGEN

QiuLishu;ZhangYonghong;Lihao;XueHaichou;LouWenxian;XuDonghong;QianZhongLi;LuPing;AndrewDeelder

1998, 16(1):  8-37. 

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AIM: To compare the efficacy of 5 test/probe systems for the detecting of schistosomal circulation antigen (SCA) in sera from infected rabbits. METHODS: Nine rabbits were infected with monosexual cercariae (either male or female), 7 rabbits were infected with bisexual cercariae as controls. Blood samples were collected periodically post infection (p.i). 15 rabbits were infected each with 250 bisexual cercaria and divided into 3 groups,2 groups were treated with allyl thiourea (295-590 mg/kg) from 19 d o...


OBSERVATION ON THE FACTORS INFLUENCING INFECTION RATE OF MERIONES UNGUICULATUS WITH BRUGIA MALAYI 

XuDagang;WanQihui;LiJie;NieWenqing

1998, 16(1):  9-41. 

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AIM: To explore the factors influencing the infection rate of M.unguiculatus with B.malayi. METHODS: The survival rate, infection rate and infection intensity were observed in M.unguiculatus infected with the infective larvae of B.malayi contained in normal saline, antibiotic, glucose or culture medium RPMI1640, respectively. RESULTS: The survival rate, infection rate and rate of infection intensity in normal saline group, antibiotic group, glucose group and RPMI1640 group were 80 9%,60 5%, and 43 ...


PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

PanSaiyi;ZhouJiexian;HuangJiankang;HuangZhibiao;RuanCaiwen;PanPo

1998, 16(1):  10-44. 

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AIM:To develope and identify monoclonal antibody(McAb) against adult Clonorchis sinensis. METHODS:BALB/c mice were immunized with soluble antigen of adult Clonorchis sinensis ,the spleen cells of immunized mice were fused with myeloma cell line SP2/0.Then the hybridoma cell lines secreting high titer of McAbs were screened.The Ig subtypes and titer of the McAbs were assayed with ELISA.The antigens recognized by the McAbs were examined with ...


新记录

A NEW SPECIES OF ANOPHELES HYRCANUS GROUP FROM NEI MONGOL AUTONOMOUS REGION (DIPTERA:CULICIDAE

XuJinjiang;LuoXinfu

1998, 16(1):  11-52. 

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Morphology of Anopheles (Anopheles) hailarensis, a new species belonging to the Anopheles hyrcanus group,collected from Hailaer,Nei Mongol Autonomous Region,P.R.of China,is described in adult,pupal,larval and egg stages.It is closely related to Anopheles sinensis Wiedemann,1828 and Anopheles heiheensis Ma,1981 .Comparisons on adult morphology among above three species and egg morphology between the new species and Anopheles sinensis ...


防治研究

STUDIES ON EXTENSIVE APPLICATION OF BROMOACETAMIDE IN VARIOUS TYPES OF SCHISTOSOMIASIS ENDEMIC AREA

ZhuDapeiYinJianyeBaoZipingCaiLiqun;

1998, 16(1):  12-57. 

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AIM:To provide scientific basis for the popularization and application of molluscide bromoacetamide.METHODS:Large scale application of bromoacetamide in twenty eight counties of four provinces was investigated and its molluscicidal effect in marshland and influence on marshland grass (Cyperus) and fishes were observed.RESULTS:High molluscicidal effect of bromoacetamlde was found against both snails and snail eggs in various environments.According to t...


实验研究

EXPERIMENTAL STUDY ON DETECTION OF SCHISTOSOMA JAPONICUM 5D GENE BY USING PCR

ChenYiping;WengXinhua;XuZhaoyue

1998, 16(1):  13-61. 

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AIM:To search for an effective method to diagnose the schistosomiasis and evaluate the effect of treatment.METHODS:A PCR protocol was designed to detect the Schistosoma japonicum 5D gene encoding an immunogenic miracidial antigen in cercariae、eggs and adult worms.RESULTS:Specific amplification of a 262 bp DNA fragment was achieved by using PCR and a 165 bp DNA fragment by using nest PCR in cercariae、adult worms and eggs of Schistosoma j...


PHENOL RED METHOD FOR MEASURING THE pH OF THE GUT CONTENTS IN LUTZOMYIA LONGIPALPIS (PSYCHODIDAE:DIPTERA)

YinshanTang

1998, 16(1):  14-67. 

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AIM:To develop a method to estimate the pH of the gut contents of female sandflies using a microcapillary feeding technique.METHODS:Female Lutzomyia longipalpis were fed with a small quantity of phenol red solution (indicator range pH 6.8 8.4) before and after a bloodmeal.The colour patterns of the gut contents were recorded by video microscopy immediately after the alimentary canal was dissected out of the sandfly body,and used to deter...