Abstract: AIM: To present a new malaria diagnostic method based on detection of malaria parasite DNA by PCR ELISA. METHODS: According to the conserved sequence of Plasmodium SSUrRNA genes reported, a pair of primers in which one primer was biotinylated and another was unbiotinylated, suitable for DNA amplification of both falciparum and vivax malaria parasites were designed and synthesized. After denaturation and washing, the incoporated biotinylated product with avidin coated on plates previously was hyb...

Key words: PCR ELISA, malaria parasite, DNA detection