PHENOL RED METHOD FOR MEASURING THE pH OF THE GUT CONTENTS IN LUTZOMYIA LONGIPALPIS (PSYCHODIDAE:DIPTERA)

›› 1998, Vol. 16 ›› Issue (1): 14-67.

• 实验研究 • Previous Articles Next Articles

PHENOL RED METHOD FOR MEASURING THE pH OF THE GUT CONTENTS IN LUTZOMYIA LONGIPALPIS (PSYCHODIDAE:DIPTERA)

Yinshan Tang

Nestor Aez Facultad de Ciencias; Universidad de Los Andes; Merida 5101; Venezuela Paul A.Bates Liverpool School of Tropical Medicine; Pembroke Place; Liverpool L3 5QA; UK;

Received:1900-01-01 Revised:1900-01-01 Online:1998-02-28 Published:1998-02-28

Abstract

Abstract: AIM:To develop a method to estimate the pH of the gut contents of female sandflies using a microcapillary feeding technique.METHODS:Female Lutzomyia longipalpis were fed with a small quantity of phenol red solution (indicator range pH 6.8 8.4) before and after a bloodmeal.The colour patterns of the gut contents were recorded by video microscopy immediately after the alimentary canal was dissected out of the sandfly body,and used to deter...

Key words: pH detection, Psychodidae, Lutzomyia, sandfly gut, phenol red, Leishmania

YinshanTang. PHENOL RED METHOD FOR MEASURING THE pH OF THE GUT CONTENTS IN LUTZOMYIA LONGIPALPIS (PSYCHODIDAE:DIPTERA)[J]. , 1998, 16(1): 14-67.

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PHENOL RED METHOD FOR MEASURING THE pH OF THE GUT CONTENTS IN LUTZOMYIA LONGIPALPIS (PSYCHODIDAE:DIPTERA)

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