中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (5): 292-296.

• 论著 • 上一篇    下一篇

云南省恶性疟原虫对青蒿素类药物及咯萘啶与氯喹敏感性的体外测定

杨恒林1; 刘德全2; 黄开国1; 董莹1; 杨品芳1; 杨亚明1; 廖明铮3; 张春勇2; 刘瑞君2   

  1. 1 云南省疟疾防治研究所; 2 中国预防医学科学院寄生虫病研究所; 3 云南省瑞丽市防疫站
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-10-30 发布日期:1997-10-30

IN VITRO SENSITIVITY OF PLASMODIUM FALCIPARUM TO DERIVATIVESOF ARTEMISININ, PYRONARIDINE AND CHLOROQUINE IN YUNNAN

Yang Henglin1; Liu Dequan2; Huang Kaiguo1; Dong Ying1; Yang Yaming1; Yang
Pinfang1; L iao Mingzheng3; Zhang Chunyong2; Liu Ruijun2   

  1. 1 Institute of Malaria Prevention and Treatmentof Yunnan Province; Simao665000 2 Institute of Parasistic Diseases, Chinese Academy of Preventive Medicine, Shanghai 2000253 Ruili City Antiepidemic Station, Ruili 678600
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-10-30 Published:1997-10-30

摘要: 目的 :了解恶性疟原虫对青蒿素类药物及咯萘啶与氯喹等抗疟药的敏感性。方法 :采用Rieckmann体外微量法检测。结果 :滇东南、滇南、老挝、滇西及缅甸恶性疟原虫对氯喹的抗性率为79%— 96% ,半数抑制量 ( ID50 )为 12 2— 2 4 0 nmol/L;对咯萘啶的抗性率为 2 0 %— 35% ,ID50 为16— 32 nmol/L ;来自老挝感染的恶性疟原虫对青蒿素类药物 (青蒿琥酯 ,还原青蒿素、蒿乙醚、蒿甲醚 )均敏感 ,其他各地抗性率为5%— 16%。结论 :云南恶性疟原虫对氯喹普遍存在抗性 ,大部分原虫对青蒿素类药物及咯萘啶敏感 ,但也有部分原虫产生了低度抗性。

关键词: 恶性疟原虫, 体外敏感性测定, 青蒿素衍生物, 咯萘啶, 氯喹

Abstract: AIM:To examine the sensitivity of Plasmodium falciparum to six antimalarials in
south Yunnan in 1991—1993.METHODS:Rieckmanns in vitro microtechnique was used.The responses of P.falciparum to chloroquine,pyronaridine and derivatives of artemisinin including artesunate, dihydroartemisinin,arteether and artemether were assayed. RESULTS:The resistance rates
of P.falciparum from southeast,west and south Yunnan,Laos,Myrnmar were 79%— 96 % ,and ID50 were
122— 240 nmol/L to chloroquine; whereas the resistance rates were 20% —35% , and ID50 were 16—32 nmol/L to pyronaridine. The resistance rates were 5% —16% to the derivatives of artemisinin, while P.falciparum from Laos showed high sensitivity to the derivatives of artemisinin. CONCLUSION: The resistance of P. falciparum to chloroquine were popular in Yunnan, most of the isolates were sensitive to the above-mentioned derivatives of artemisinin and pyronaridine, only a few isolates exhibited
resistance.

Key words: Plasmodium falciparum, in vitro test, derivatives of artem isinin, pyronaridine, chloroquine