Determination of Free Thiols in the Chimeric Protein PfCP-2.9 of Plasmodium faldparum

Abstract

Abstract: 　Objective To determine the free thiols in the chimeric protein PfCP-2. 9 of Plasmodium falciparum expressed by Pichia pastoris. Methods Two experiments of reverse phase HPLC and Ellman' s reaction were applied to the PfCP-2.9 for the determination of its free thiols. For RP-HPLC analysis, three kinds of samples were tested: PfCP-2. 9, dithiothreitol-reduced PfCP-2.9 and indoacetic acid-alkylated PfCP-2.9. Results Both experiments showed that there were no any free thiols present in the PfCP-2. 9. Conclution The disulfide bonds between cysteine residues of PfCP-2. 9 were formed completely.

Key words: Plasmodium falciparum, chimeric protein, reverse phase HPLC, Ellman' reaction, free thiol

QIANFeng;PANWei-qing. Determination of Free Thiols in the Chimeric Protein PfCP-2.9 of Plasmodium faldparum[J]. , 2003, 21(2): 11-101.

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Determination of Free Thiols in the Chimeric Protein PfCP-2.9 of Plasmodium faldparum

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