Investigation of Pomacea canaliculata and rodent densities and their infection status with Angiostrongylus cantonensis in Nan′ao Island of Guangdong Province

Abstract

Abstract: Objective To investigate the densities of Pomacea canaliculata and rodents, as well as their infection status with Angiostronglyus cantonensis in Nanao Island of Guangdong Province, China. Methods Stratified random sampling was made in three villages（Gongqian village, Jinshan village and Liudu village） in Nanao Island from December 2015 to September 2016. P. canaliculata and rodents were collected and the sampling sites were mapped by GPS. For P. canaliculata, morphological observation was performed, followed by DNA extraction and PCR to double-check the species. Infection in P. canaliculata was examined by lung-microscopy, tissue homogenate and enzyme digestion. For rodents, morphological observation was first performed to identify species and sex, and the species was further confirmed by PCR on DNA from the liver tissue. The heart and lung were dissected to inspect A. cantonensis infection. Morphological examination and PCR were performed to determine the species of the collected samples of parasite. Data were analyzed by using SAS9.3 software. Results Totally 2 192 P. canaliculata were collected and 1 190 were selected for examination. Of them 72（6.1%） were positive for infection. 110 rodents were captured, consisting of the species of Rattus norvegicus, R. flavipectus, R. losea and Suncus murinus. Of them 32（29.1%） were positive for infection, consisting of 31 R. norvegicus and one R. flavipectus. The other two species showed negative results. As expected, the length of COⅠ amplification products was 670 bp and 706 bp, respectively, for P. canaliculata and rodents, while the length of ITS2 for A. cantonensis was 693 bp. There was a significant difference in density（H = 11.603 5, P < 0.01） and infection rate（χ² = 65.144 1, P < 0.01） in P. canaliculata among different seasons. There was also a significant seasonal difference in the density of rats（H = 19.268 2, P < 0.01）. P. canaliculata from rivers had the highest density （8.8/m²）, while those from irrigation canals and ditches showed the highest infection rate（7.5%）. There was no significant difference in the density of P. canaliculata inhabiting in different breeding environments （H = 3.909 3, P > 0.05）. The infection rates for P. canaliculata and rodents were both closely related to the distance from breeding grounds to residential areas, the closer the distance, the higher the infection rate. Rats with heavier weight had higher infection rate（χ² = 17.530 4, P < 0.01）. Conclusion P. canaliculata as the intermediate host and rats as the definitive host of A. cantonensis are widely distributed in Nanao Island and harbor different degrees of infection.

Key words: Angiostronglyus cantonensis, Pomacea canaliculata, Rat, Density, Infection status, Nanao Island

CLC Number:

R383.1

HU Qiu-an, ZHANG Yi, GUO Yun-hai, LV Shan, XIA Shang, LIU He-xiang. Investigation of Pomacea canaliculata and rodent densities and their infection status with Angiostrongylus cantonensis in Nan′ao Island of Guangdong Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(3): 239-245.

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References 34

[1]	Graeff-Teixeira C, Da SA, Yoshimura K.Update on eosinophilic meningoencephalitis and its clinical relevance[J]. Clin Microbiol Rev, 2009, 22(2): 322-348.
[2]	Barratt J, Chan D, Sandaradura I, et al. Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen[J]. Parasitology, 2016, 143(9): 1087-1118.
[3]	Yang TB, Wu ZD, Lun ZR.The apple snail Pomacea canaliculata, a novel vector of the rat lungworm, Angiostrongylus cantonensis: its introduction, spread, and control in China[J]. Hawaii J Med Public Health, 2013, 72(6 Suppl 2): 23-25.
[4]	Lv S, Zhang Y, Steinmann P, et al. Emerging angiostrongyliasis in Mainland China[J]. Emerg Infect Dis, 2008, 14(1): 161-164.
[5]	Song L, Wang X, Yang Z, et al. Angiostrongylus cantonensis in the vector snails Pomacea canaliculata and Achatina fulica in China: a meta-analysis[J]. Parasitol Res, 2016, 115(3): 913-923.
[6]	GB/T 28940-2012 病媒生物感染病原体采样规程鼠类[S]. 北京:中华人民共和国卫生部中国国家标准化管理委员会,2012.
[7]	GB/T 23798-2009 病媒生物密度监测方法鼠类[S]. 北京:中华人民共和国国家质量监督检验检疫总局, 2009.
[8]	胡自强, 胡运瑾. 福寿螺的形态构造[J]. 动物学杂志, 1991, 26(5): 4-6.
[9]	宋红梅, 胡隐昌, 王培欣, 等. 福寿螺线粒体DNA COⅠ基因序列测定及分类地位[J]. 动物学杂志, 2010, 45(1): 1-7.
[10]	马雪莲, 谭峰, 潘长旺. 广州管圆线虫中间宿主福寿螺感染检测方法的比较[J]. 中国病原生物学杂志, 2008, 3(2): 130-132.
[11]	刘和香, 张仪, 吕山, 等. 三种方法检测福寿螺肺囊内广州管圆线虫效果的比较研究[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(1): 53-56.
[12]	郑剑宁, 周力沛, 裘炯良, 等. 亚洲10国鼠科常见鼠种类、分布与鉴定概述[J]. 中国媒介生物学及控制杂志, 2008, 19(3): 261-263.
[13]	刘润吉. DNA条形码检测技术在媒介鼠类鉴定中的研究与示范应用[D]. 安徽: 安徽理工大学, 2014.
[14]	Lv S, Zhang Y, Liu HX, et al. Angiostrongylus cantonensis: morphological and behavioral investigation within the freshwater snail Pomacea canaliculata[J]. Parasitol Res, 2009, 104(6): 1351-1359.
[15]	王菲, 曹淑祯, 张霄霄, 等. 广州管圆线虫生长发育及形态特征研究现状[J]. 国际医学寄生虫病杂志, 2013, 40(4): 225-230.
[16]	王鸽. 广西蛞蝓感染广州管圆线虫情况分析及三地广州管圆线虫亲缘关系探索[D]. 广西: 广西医科大学, 2012.
[17]	霍雪霞, 陈戊申, 古伟志, 等. 不同生态环境中螺广州管圆线虫感染率的调查[J]. 实用预防医学, 2012, 19(7): 1010-1012.
[18]	林国华, 黄明松, 程由注, 等. 福建省龙海市广州管圆线虫贝类宿主种群生态及感染率调查研究[J]. 中国人兽共患病学报, 2014, 30(8): 821-827.
[19]	Zhang RL, Chen MX, Gao ST, et al. Enzootic angiostrongy-liasis in Shenzhen, China[J]. Emerg Infect Dis, 2008, 14(12): 1955-1956.
[20]	柯雪梅, 苏畅, 王秀芹. 2010~2014年厦门市福寿螺广州管圆线虫感染状况调查[J]. 中国当代医药, 2015, 22(35): 157-159.
[21]	Ximin H, Jianwei D, Chongjin T, et al. Epidemic status of Angiostrongylus cantonensis in Hainan Island, China[J]. Asian Pac J Trop Med, 2011, 4(4): 275-277.
[22]	李天美, 方文, 李科荣, 等. 2013-2014年大理市广州管圆线虫感染状况监测结果[J]. 寄生虫病与感染性疾病, 2016, 14(2): 67-70.
[23]	陈楚炫, 何惠芳, 殷竹, 等. 广州市番禺地区褐云玛瑙螺和福寿螺广州管圆线虫感染情况调查[J]. 中国血吸虫病防治杂志, 2012, 24(3): 336-338.
[24]	Simoes RO, Maldonado JA, Olifiers N, et al. A longitudinal study of Angiostrongylus cantonensis in an urban population of Rattus norvegicus in Brazil: the influences of seasonality and host features on the pattern of infection[J]. Parasit Vectors, 2014, 7(1): 100.
[25]	Martin-Alonso A, Foronda P, Quispe-Ricalde MA, et al. Seroprevalence of Angiostrongylus cantonensis in wild rodents from the Canary Islands[J]. PLoS One, 2011, 6(11): e27747.
[26]	Tujan MA, Fontanilla IK, Paller VG.Vectors and spatial patterns of Angiostrongylus cantonensis in selected rice-farming villages of Munoz, Nueva Ecija, Philippines[J]. Parasitol Res, 2016, 2016(2): 1-7.
[27]	黄达娜, 耿艺介, 谢旭, 等. 深圳市鼠类广州管圆线虫感染状况调查[J]. 国际医学寄生虫病杂志, 2011, 38(3): 180-182.
[28]	Yang X, Qu Z, He H, et al. Enzootic angiostrongyliasis in Guangzhou, China, 2008-2010[J]. Am J Trop Med Hyg, 2012, 86(5): 846-849.
[29]	林国华, 颜翠兰, 蔡茂荣, 等. 福建省漳州市鼠类宿主感染广州管圆线虫调查[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(2): 133-136.
[30]	耿艺介, 黄达娜, 谢旭, 等. 深圳市广州管圆线虫动物宿主感染状况调查[J]. 中华疾病控制杂志, 2011, 15(9): 802-805.
[31]	刘和香, 张仪, 周晓农, 等. 福寿螺休眠期体内广州管圆线虫生长发育及其感染性的观察研究[J]. 中国寄生虫学与寄生虫病杂志, 2006, 24(4): 269-272.
[32]	周卫川, 吴宇芬, 杨佳琪. 福寿螺在中国的适生性研究[J]. 福建农业学报, 2003, 18(1): 25-28.
[33]	尹绍武, 颜亨梅, 王洪全, 等. 福寿螺的生物学研究[J]. 湖南师范大学自然科学学报, 2000, 23(2): 76-82.
[34]	刘和香, 张仪, 周晓农, 等. 不同发育期福寿螺对广州管圆线虫易感性的实验研究[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(5): 262-265.

调查村Village	采集螺/只No. collected snails	检查螺/只No. examined snails	阳性螺/只No. infected snails	螺密度 /只·m^-2 Density of snails /No·m^-2	感染率/%Infection rate/%
宫前Gongqian	644	283	9	8.0	3.2
六都Liudu	886	486	24	9.9	4.9
金山Jinshan	662	421	39	3.5	9.3
合计Total	2 192	1 190	72	6.7	6.1

调查村Villages	回收鼠笼 /只 No. recycled cage	捕鼠/只 No. rats	阳性鼠/只No. positive rats	鼠密度 /只·笼^-1 Density of rats /No·cage^-1	感染率/%Positive rate/%
宫前Gongqian	289	71	29	0.2	40.9
六都Liudu	117	13	2	0.1	15.4
金山Jinshan	130	26	1	0.2	3.9
合计Total	536	110	32	0.2	29.1

滋生环境Environment	采集螺/只No. snails	检查螺/只No. examined snails	阳性螺/只No. infected snails	螺密度 /只·m^-2 Density of snails /No·m^-2	感染率/%Positive rate/%
河道River	788	415	23	8.8	5.5
沟渠Ditch	909	429	32	5.8	7.5
水塘Ponds	224	104	7	7.6	6.7
田园Fields	271	242	10	4.5	4.1
合计Total	2 192	1 190	72	6.7	6.1

栖息环境Environment	回收鼠笼 /只 No. recycled cages	捕鼠/只 No. rats	阳性鼠/只No. positive rats	鼠密度 /只·笼^-1 Density of rats /No·cage^-1	感染率/%Infection rate/%
野外田园Fields	111	12	2	0.1	16.7
河沟边Waterside	111	22	5	0.2	22.7
居民区Residential area	199	43	8	0.2	18.6
垃圾废品回收站Garbage collection station	115	33	17	0.3	51.5
合计Total	536	110	32	0.2	29.1

离居民区距离/m Distance from residential areas/m	采集螺/只No. snails	检查螺/只No. examined snails	阳性螺/只No. infected snails	螺密度 /只·m^-2 Density of snails /No·m^-2	感染率/%Positive rate/%
< 10	976	518	41	7.1	7.9
10~50	466	292	21	4.5	7.2
51~200	679	339	9	9.6	2.7
> 200	71	41	1	6.9	2.4
合计Total	2 192	1 190	72	6.7	6.1