›› 2005, Vol. 23 ›› Issue (2): 4-81.

• 论著 • Previous Articles     Next Articles

Comparison of PCR and Isoenzyme Analysisin Identification of Anopheles minimus A and C

ZHENG Bin;TANG Lin-hua;MA Ya-jun;WANG Xue-zhong;ZHOU Shui-sen;SHI Wen-qi   

  1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention,WHO Collaborating Center for Malaria, Schistosomiasis, Shanghai 200025, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-04-30 Published:2005-04-30

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Abstract: Objective To compare the PCR and isoenzyme methods in identification of Anopheles minimus A and C. Methods Samples of An. minimus collected in the field were identified from An. aconitus and An. jeyporiensis by PCR-RFLP after morphological examination, then classified by PCR-ASA. The mosquitoes identified by this method were further analyzed for isoenzyme to observe the difference, which was previously used as a technique to classify An. minimus A and C. Results PCR method can differentiate An.minimus A and C simply and quickly. For Isoenzyme method, only EST allozyme pattern displayed difference. Conclusion PCR method is better than isoenzyme analysis in differentiating An. minimus A and C.

Key words: PCR, Isoenzyme, Anopheles minimus, Iidentification