Abstract: Objective To screen cDNA library from Clonorchis sinensis for gene identification， and to clone and construct library of secreted recombinant proteins. Methods cDNA library from Clonorchis sinensis was screened for identifying new genes by Blastn protocol， the sequence of which was further analyzed by Motifscan and NCBI Conserved Domain Search protocol. Results The secreted proteins （Pcs004f03） were identified， with a 689 bp DNA sequence （187aa）， and a theoretical molecular weight of Mr 21 100. It is predicted that the gene CsCrSP contains one N-glycosylation site， three N-myristoylation sites， three casein kinase II phosphorylation sites， one cAMP-and cGMP-dependent protein kinase phosphorylation site and protein kinase C phosphorylation site， a signal peptide at N-terminals and SCP-like extracellular protein domain. Conclusion The CsCrSP gene is a secretory cysteine-rich protein with a SCP-like extracellular protein domain.

Key words: Clonorchis sinensis, Secreted protein, Cloning