›› 2007, Vol. 25 ›› Issue (1): 14-64.

• 防治研究 • Previous Articles     Next Articles

Asymptomatic Leishmania Infection in Human Population of Wenxian County, Gansu Province

WANG Jun-yun1;FENG Yu2;GAO Chun-hua1;JIN Chang-fa1;CHEN Sheng-bang2;ZHANG Chou-ji2;HE Jin-ping2;YANG Chen-ming2;YANG Yue-tao1;BAO Yi-fang1   

  1. 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China； 2 Department of Parasitic Disease Control, Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730020, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-28 Published:2007-02-28

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Abstract: Objective To analyze the status of Leishmania infantum asymptomatic infection in human population of a Kala-azar endemic area in Wenxian County, Gansu Province, and to evaluate the tests used. Methods Blood samples were tested by PCR using two pairs of primers, RV1-RV2 and K13A-K13B, for detecting Leishmania-specific DNA. ELISA and rK39-dipstick were used to detect Leishmania-specific antibodies. Results The positive rate of PCR, ELISA and rK39-dipstick was 30.9%（83/269), 24.2%（65/269） and 0(0/269) respectively. Conclusion The prevalence of asymptomatic infection of L. infantum in humans is high in the area. PCR test based on RV1-RV2 and K13A-K13B primer pairs is a sensitive and specific method for detecting the asymptomatic infection.

Key words: Leishmania infantum, Asymptomatic infection, PCR, ELISA, rK39-dipstick