PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

›› 1998, Vol. 16 ›› Issue (1): 10-44.

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PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

Pan Saiyi; Zhou Jiexian; Huang Jiankang; Huang Zhibiao; Ruan Caiwen; Pan Po

Institute of Parasitic Diseases of Guangdong Province; Guangzhou; 510300;

Received:1900-01-01 Revised:1900-01-01 Online:1998-02-28 Published:1998-02-28

Abstract

Abstract: AIM:To develope and identify monoclonal antibody(McAb) against adult Clonorchis sinensis. METHODS:BALB/c mice were immunized with soluble antigen of adult Clonorchis sinensis ,the spleen cells of immunized mice were fused with myeloma cell line SP2/0.Then the hybridoma cell lines secreting high titer of McAbs were screened.The Ig subtypes and titer of the McAbs were assayed with ELISA.The antigens recognized by the McAbs were examined with ...

Key words: Clonorchis sinensis, McAb, hybridoma cell lines

PanSaiyi;ZhouJiexian;HuangJiankang;HuangZhibiao;RuanCaiwen;PanPo. PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS[J]. , 1998, 16(1): 10-44.

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PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

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