PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

›› 1998, Vol. 16 ›› Issue (1): 10-44.

• 论著 • Previous Articles Next Articles

PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

Pan Saiyi; Zhou Jiexian; Huang Jiankang; Huang Zhibiao; Ruan Caiwen; Pan Po

Institute of Parasitic Diseases of Guangdong Province; Guangzhou; 510300;

Received:1900-01-01 Revised:1900-01-01 Online:1998-02-28 Published:1998-02-28

Abstract

Abstract: AIM:To develope and identify monoclonal antibody(McAb) against adult Clonorchis sinensis. METHODS:BALB/c mice were immunized with soluble antigen of adult Clonorchis sinensis ,the spleen cells of immunized mice were fused with myeloma cell line SP2/0.Then the hybridoma cell lines secreting high titer of McAbs were screened.The Ig subtypes and titer of the McAbs were assayed with ELISA.The antigens recognized by the McAbs were examined with ...

Key words: Clonorchis sinensis, McAb, hybridoma cell lines

PanSaiyi;ZhouJiexian;HuangJiankang;HuangZhibiao;RuanCaiwen;PanPo. PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS[J]. , 1998, 16(1): 10-44.

[1]	WU Yinjuan, YI Xueqing, LI Meiyu, XU Anyuan, WU Aoxun, ZHONG Zifeng, LI Xuerong. Effect and mechanism of Csi-miR-125a induced liver fibrosis in the exosomes of Clonorchis sinensis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 198-204.
[2]	WU Aoxun, ZHONG Zifeng, XU Anyuan, PAN Xiaowen, YI Xueqing, WU Yinjuan, LI Xuerong. Research progress on the mechanism of Clonorchis sinensis miRNAs in the initiation and progression of cholangiocarcinoma [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 269-273.
[3]	CAI Zezheng, YANG Ping, LIAO Qianqian. A child case of complicated Paragonimus infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(6): 813-816.
[4]	LAN Weiming, ZENG Yufeng, QIU Tingting, ZHOU Changhai, CHEN Zhe, GE Jun, ZENG Xiaojun, ZHU Tingjun. Analysis of the current status of Clonorchis sinensis infection in Xinfeng County, Jiangxi Province from 2020 to 2022 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(5): 659-663.
[5]	CHAI Yingzhi, CHEN Hualiang, YU Kegen, ZHANG Xuan, WANG Xiaoxiao, ZHANG Jiaqi, XU Wenjie, RUAN Wei. Analysis on surveillance results of clonorchiasis in Zhejiang Province from 2013 to 2022 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 469-474.
[6]	WANG Jufeng, XUE Shijie, LIU Jiangyong, LAI Dehua, LUN Zhaorong. Infection and identification of Clonorchis sinensis metacercariae in Pseudorasbora parva in multiple regions of China [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 475-480.
[7]	LIU Liu, ZHANG Jing, LI Jianke, ZHANG Hao, ZHANG Fengyu. Distribution of the neurotransmitter 5-hydroxytryptamine in Clonorchis sinensis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 78-82.
[8]	ZHAO Lei, LI Jia, MO Gang, LI Chun, HUANG Guoyang, PENG Xiaohong. Effect of Clonorchis sinensis infection on hepatic fibrosis and immune regulation in mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 760-765.
[9]	LI Xiaoqin, LAI Yashi, CHEN Yu, LV Jiahui, WEI Shuai, ZHANG Lilin, HE Shanshan, SHI Yunliang, LI Yanwen. Ultrastructural observation on excystment of metacercaria of Clonorchis sinensis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 601-608.
[10]	XU Yin, LIU Ting, XU Hui, ZENG Xiaojun, LAN Weiming, GONG Zhihong, DAI Kunjiao, QIU Tingting, HAO Xian, XIE Shuying. Establishment and application of PCR-CRISPR/Cas12a-based detection method for Clonorchis sinensis metacercaria [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 421-426.
[11]	HUANG Guang-hua, ZHANG Bo, LAI Hai-tao, Lv Guo-li, LIN Yuan, TANG Wen-qian, WAN Xiao-ling, JIANG Zhi-hua, LIU Jian. Analysis on the prevalence of Clonorchis sinensis infection in Lingshan County, Guangxi from 2016 to 2019 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 673-676.
[12]	WANG Ting, YANG Qing-li, LENG Jing, LI Bao-ying, SHEN Ji-qing, DAI Yue. Expression of the high mobility group box 1 homologous protein of Clonorchis sinensis and its effect on nuclear transcription factor-κB activation in mouse macrophages [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 305-308.
[13]	TANG Lei, YUAN Shuang, YIN Shi-hui, GE Tao, GE Jing-xue, XING Zhi-feng. Investigation on the prevalence of Clonorchis sinensis infection in human population of Heilongjiang Province, 2015 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(6): 741-745.
[14]	CHEN Bao-jian, XIE Han-guo, XIE Xian-liang, JIANG Dian-wei, CHEN Yun-hong, GAO Lan-lin. Investigation of Clonorchis sinensis infection in the national surveillance site of Pucheng County, Fujian Province during 2016—2020 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(5): 716-719.
[15]	LI An-mei, HUANG Yu-ting, YAO Dan-cheng, ZHU Ai-ya, LI Yang, SHI Wei-fang, DAI Jia-rui, GENG Yan, SHE Dan-ya, ZHANG Nian-heng. Investigation and analysis on the prevalence of Clonorchis sinensis infection in Guizhou Province in 2015 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(1): 125-128.

PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CLONORCHIS SINENSIS

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments