AIM: To construct a cDNA expression library from erythrocytic Plasmodium falciparum (FCC/HN). METHODS: 1224 μg total RNA and 35.84 μg poly (A)⁺ mRNA have been successively obtained from the blood-stage Plasmodium falciparum mainly consisting of trophozoites using“single-step method”and by chromatography on oligo-(dT) cellulose. With the reverse transcriptase (M-MLV ) , 10 μg mRNA was synthesized into 1.75 μg blunt cDNA. After the cDNA was ligated to EcoRI (NotI, SalI) adapter and purified by fractionation, 200 ng cDNA of about 500 bp - 7 kb was collected. Of which 50 ng cDNA was ligated into λgt11 phage particles and was packaged with the packagen extract system in vitro. This library was characterized primarily by PCR. RESULTS: A cDNA library containing 10⁶ recombinants has been constructed. CONCLUSION: The capacity of this library and the size of cDNA is suitable for further study.