Abstract: 　AIM:To determine the nucleotide sequence of the partial gene of the histidine rich proteinⅡ (HRPⅡ) the Plasmodium falciparum PFD 3/YN and find out the differences of the HRPⅡ gene sequence between this isolate and other isolates.METHODS:The histidine rich protein Ⅱ gene fragment was amplified by polymerase chain reaction and cloned into M13 bacteriophage.M13 HRPⅡ single strand DNAs of three positive clones were extracted, respectively.The nucleotide sequence of the HRPⅡ gene fragment was determined by the dideoxy chain termination method.Using PCGENE software to compare and analyze the HRPⅡ gene sequence among the different isolates.RESULTS: Different degrees of diversity of the HRPⅡgene sequences were found among Plasmodium falciparum PFD 3/YN(from China)and two other isolates(7G8 from Brazil and D10 from Gambia).The HRPⅡ in the three isolates exhibited 70.3% homology in amino acid sequences and 68.6% homology in the nucleic acid sequences.CONCLUSION:There were differences in HRPⅡ gene sequence among the Plasmodium falciparum PFD 3/YN and two other isolates(7G8 and D10).

Key words: Plasmodium falciparum, histidine rich proteine Ⅱgene, gene sequencing