Abstract: 　Objective] To prepare and characterize the monoclonal antibodies(McAbs) against lactate dehydrogenase of the Plasmodium falciparum(LDHp). [Methods] BALB/c mice were immunized with purified recombinant LDHp and McAbs against LDHp were prepared according to the protocol of hybridoma technique. The McAbs were characterized by ELISA and Western blot analysis. [Results] Two McAbs against LDHp antigen were obtained. Both McAbs were IgG 2b . The titer of two McAbs(2A5,1H10) in the ascites was \{1∶25 600\} and \{1∶12 800\}, and in supernatant were \{1∶512\},\{1∶256\} respectively. The result of ELISA indicated that two McAbs reacted only with P.falciparum, and did not react with normal human red blood cells, P.vivax, Toxoplasma gondii, Schistosoma japonicum. It is recognized 33 kDa protein which was defined as LDHp by Western blot analysis. [Conclusion] Two hybridoma cell lines secreting high titer of McAbs against LDHp with high specificity were established.

Key words: Plasmodium falciparum, lactate dehydrogenase, monoclonal antibodies